Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor

被引：40

作者：

Cane, DE ^{[1
]}

Chow, C ^{[1
]}

Lillo, A ^{[1
]}

Kang, I ^{[1
]}

机构：

[1] Brown Univ, Dept Chem, Providence, RI 02912 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2001年 / 9卷 / 06期

关键词：

D O I：

10.1016/S0968-0896(01)00050-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mevalonate-independent biosynthetic pathway to isopentenyl diphosphate and dimethylallyl diphosphate, the universal precursors to the isoprenoids, operates in eubacteria, including Escherichia coli, in algae, and in the plastids of higher plants. A search of the Sanger Centre Streptomyces coelicolor genome database revealed open reading frames with ca. 40-50% identity at the deduced amino acid level to the first three E. coli enzymes of this pathway, corresponding to deoxyxylulose phosphate synthase, deoxyxylulose phosphate reductoisomerase and 2-C-methyl erythritol 4-phosphate cytidylyltransferase. The S. coelicolor genes have been cloned and expressed in E. coli, and the recombinant proteins characterized physically and kinetically. The presence of the corresponding enzyme activities in extracts of S. coelicolor CH999 further supports the operation of the mevalonate-independent pathway in this organism. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：1467 / 1477

页数：11

共 49 条

[1]

[Anonymous], 1999, COMPREHENSIVE NATURA, DOI DOI 10.1016/B978-0-08-091283-7.00043-6

[2]

ARCAMONE F, 1970, GAZZ CHIM ITAL, V100, P581

[3]

ARIGONI D, UNPUB

[4]

Cane D. E., 1999, Comprehensive Natural Products Chemistry, P155, DOI [DOI 10.1016/B978-0-08-091283-7.00039-4, 10.1016/B978-0-08-091283-7.00039-4]