Cloning and characterization of a novel transforming growth factor-β1-induced TIAF1 protein that inhibits tumor necrosis factor cytotoxicity

被引：33

作者：

Chang, NS ^{[1
]}

Mattison, J ^{[1
]}

Cao, H ^{[1
]}

Pratt, N ^{[1
]}

Zhao, Y ^{[1
]}

Lee, C ^{[1
]}

机构：

[1] Guthrie Med Ctr, Lab Mol Immunol, Guthrie Res Inst, Sayre, PA 18840 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 253卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.9846

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine how TGF-beta 1 protects L929 fibroblasts against TNF-alpha cytotoxicity, we report the isolation and characterization of a novel cDNA encoding a 12-kDa TGF-beta 1-induced antiapoptotic factor, designated TIAF1. GFP-tagged TIAF1 protein is present mostly in perinuclear and nuclear locations. TIAF1 inhibits the cytotoxic effects of TNF-alpha and overexpressed TNF receptor adaptors TRADD, FADD, and RIP. L929 stable transfectants expressing TIAF1 do not have significant changes in the expression of TNF receptors and effector or regulatory proteins in apoptosis, which may account for the acquired TNF resistance in these cells. Notably, these cells have a significantly suppressed I kappa B-alpha protein expression, and I kappa B-alpha degradation is blocked when exposing these cells to TNF-alpha. Similarly, stimulation of untransfected L929 cells with TGF-beta 1 results in suppression of I kappa B-alpha expression and retarded I kappa B-alpha degradation in response to TNF-alpha. Despite the fact that the mechanism for blocking TNF cytotoxicity is unknown, TIAF1 is apparently involved in TGF-beta 1 inhibition of I kappa B-alpha expression and suppression of TNF-mediated I kappa B-alpha degradation. (C) 1998 Academic Press.

引用

页码：743 / 749

页数：7

共 31 条

[1] TGF beta 1 inhibits NF-kappa B/Rel activity inducing apoptosis of B cells: Transcriptional activation of I kappa B alpha
Arsura, M
Wu, M
Sonenshein, GE
[J]. IMMUNITY, 1996, 5 (01) : 31 - 40
[2] I-KAPPA-B - A SPECIFIC INHIBITOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR
BAEUERLE, PA
BALTIMORE, D
[J]. SCIENCE, 1988, 242 (4878) : 540 - 546
[3] An essential role for NF-kappa B in preventing TNF-alpha-induced cell death
Beg, AA
Baltimore, D
[J]. SCIENCE, 1996, 274 (5288) : 782 - 784
[4] BCL-X, A BCL-2-RELATED GENE THAT FUNCTIONS AS A DOMINANT REGULATOR OF APOPTOTIC CELL-DEATH
BOISE, LH
GONZALEZGARCIA, M
POSTEMA, CE
DING, LY
LINDSTEN, T
TURKA, LA
MAO, XH
NUNEZ, G
THOMPSON, CB
[J]. CELL, 1993, 74 (04) : 597 - 608
[5] Tumor necrosis factor receptor (TNFR)-associated factor 2A (TRAF2A), a TRAF2 splice variant with an extended RING finger domain that inhibits TNFR2-mediated NF-κB activation
Brink, R
Lodish, HF
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (07) : 4129 - 4134
[6] Regulation of tumor necrosis factor- and fas-mediated apoptotic cell death by a novel cDNA, TR2(L)
Cao, H
Mattison, J
Zhao, Y
Joki, N
Grasso, M
Chang, NS
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 227 (01) : 266 - 272
[7] Cloning and characterization of a transforming growth factor β1-induced anti-apoptotic adhesion protein TIF2
Carey, GB
Chang, NS
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (01) : 283 - 286
[8] Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-β1
Chang, NS
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 273 (06): : C1987 - C1994
[9] TRANSFORMING GROWTH-FACTOR-BETA-1 INDUCTION OF NOVEL EXTRACELLULAR-MATRIX PROTEINS THAT TRIGGER RESISTANCE TO TUMOR-NECROSIS-FACTOR CYTOTOXICITY IN MURINE L929 FIBROBLASTS
CHANG, NS
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (13) : 7765 - 7772
[10] CHANG NS, 1998, IN PRESS INT J MOL M

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