Separate and simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

被引：27

作者：

Olsson, L. ^{[1
]}

Soerensen, H. R. ^{[2
]}

Dam, B. P. ^{[1
]}

Christensen, H. ^{[1
]}

Krogh, K. M. ^{[1
]}

Meyer, A. S. ^{[1
]}

机构：

[1] Tech Univ Denmark, Bioctr, DK-2800 Lyngby, Denmark

[2] Novozymes AS, DK-2880 Bagsvaerd, Denmark

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2006年 / 129卷 / 1-3期

关键词：

xylose conversion; ethanol; starch free fibers; vinasse;

D O I：

10.1385/ABAB:129:1:117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.

引用

页码：117 / 129

页数：13

共 29 条

[1] Metabolic engineering applications to renewable resource utilization [J].