Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus

被引：97

作者：

Veau, B ^{[1
]}

Courtois, M ^{[1
]}

Oudin, A ^{[1
]}

Chénieux, JC ^{[1
]}

Rideau, M ^{[1
]}

Clastre, M ^{[1
]}

机构：

[1] Univ Tours, Fac Pharm, Lab Biol Mol & Biochim Vegetale, F-37200 Tours, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1517卷 / 01期

关键词：

1-deoxy-D-xylulose 5-phosphate reductoisomerase; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; monoterpenoid indole alkaloid; 2C-methyl-D-erythritol 4-phosphate pathway; Catharanthus roseus;

D O I：

10.1016/S0167-4781(00)00240-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to l-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：159 / 163

页数：5

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