A molecular delivery system by using AFM and nanoneedle

被引：86

作者：

Han, SW

Nakamura, C

Obataya, I

Nakamura, N

Miyake, J

机构：

[1] Natl Inst AIST, RICE, Amagasaki, Hyogo 6610974, Japan

[2] Tokyo Univ Agr & Technol, Div Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 2005年 / 20卷 / 10期

关键词：

cell manipulation; gene transfer; AFM; force-distance curve; avidin-biotin unbinding force;

D O I：

10.1016/j.bios.2004.08.023

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We developed a new low invasive cell manipulation and gene or molecule transfer system in a single living cell by using an atomic force microscope (AFM) and ultra thin needle, a nanoneedle. DNA was immobilized on the surface of the nanoneedle by covalent bonding and avidin-biotin affinity binding. Immobilization of DNA on the nanoneedle was confirmed by measuring the unbinding force between avidin and biotin. The DNA-immobilized nanoneedle was successfully inserted into HEK293 cells. Though TO-PRO-3 iodide staining experiments using confocal microscopy, we observed the immobilized DNA on the surface of the nanoneedle, which was retained after 10 times insertions to and evacuations from a living cell. (c) 2004 Elsevier B.V. All rights reserved.

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收藏

页码：2120 / 2125

页数：6

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