Testosterone and food restriction modulate hepatic lycopene isomer concentrations in male F344 rats

被引:35
作者
Boileau, TWM
Clinton, SK
Zaripheh, S
Monaco, MH
Donovan, SM
Erdman, JW
机构
[1] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA
[2] Ohio State Univ, Arthur G James Canc Hosp, Columbus, OH 43210 USA
[3] Ohio State Univ, Richard J Solove Res Inst, Div Hematol & Oncol, Columbus, OH 43210 USA
关键词
lycopene; testosterone; prostate cancer; rats; antioxidants;
D O I
10.1093/jn/131.6.1746
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
We previously demonstrated that the castration of male rats profoundly increases hepatic lycopene compared with intact controls. Here we further characterized the role of testosterone in modulating hepatic lycopene accumulation and isomer patterns in male rats. Furthermore, because castration significantly decreases ad libitum food consumption, we investigated the influence of food restriction on lycopene metabolism. Forty male F344 rats 8 wk of age were randomly assigned to one of four treatments (n = 10/group): 1) intact, free access to food, 2) castration, free access to food, 3) castration plus testosterone implants, free access to food and 4) intact, 20% food restricted. All rats were fed an AIN-based diet with 0.25 g lycopene las 10% water-soluble beadlets)/kg diet for 3 wk. Serum testosterone was 5.31 +/- 1.46 nmol/L in intact controls allowed free access to food, reduced in castrated animals (0.52 +/- 0.10, P < 0.0001 versus controls) and intact, food-restricted rats (1.53 +/- 0.49 nmol/L, Pt 0.0001 versus controls) and greater (17.23 +/- 3.09 nmol/L) in castrated rats administered testosterone (P < 0.0001 versus controls). Castrated rats accumulated approximately twice as much liver lycopene (74.5 +/- 8.5 nmol/g; P < 0.01 versus controls) as intact rats allowed free access to food (39.5 +/- 5.0) despite 13% lower dietary lycopene intake (P < 0.001; 3.38 +/- 0.07 versus 3.95 +/- 0.06 mg lycopene/d). Testosterone replacement in castrated rats returned liver lycopene concentrations (32.5 +/- 5.5 nmol lycopene/g with 3.76 +/- 0.05 mg dietary lycopene/d) to those observed in intact rats. Food restriction resulted in a 20% decrease in lycopene intake but significantly increased liver lycopene by 68% (66.3 +/- 7.9 nmol lycopene/g with 3.38 +/- 0.00 mg lycopene/d) compared with controls and castrated rats administered testosterone. These results suggest that androgen depletion and 20% food restriction increase hepatic lycopene accumulation. We hypothesize an endocrine and dietary interaction, where higher androgen concentrations and greater energy intake may stimulate lycopene metabolism and degradation.
引用
收藏
页码:1746 / 1752
页数:7
相关论文
共 54 条
[1]  
*AM ASS OIL CHEM, 1984, 438 AOCS AA AM ASS O
[2]   Cis-lycopene is more bioavailable than trans-lycopene in vitro and in vivo in lymph-cannulated ferrets [J].
Boileau, AC ;
Merchen, NR ;
Wasson, K ;
Atkinson, CA ;
Erdman, JW .
JOURNAL OF NUTRITION, 1999, 129 (06) :1176-1181
[3]   Tissue lycopene concentrations and isomer patterns are affected by androgen status and dietary lycopene concentration in male F344 rats [J].
Boileau, TWM ;
Clinton, SK ;
Erdman, JW .
JOURNAL OF NUTRITION, 2000, 130 (06) :1613-1618
[4]   SUSTAINED SUPPRESSION OF TESTOSTERONE PRODUCTION BY THE LUTEINIZING-HORMONE RELEASING-HORMONE AGONIST BUSERELIN IN PATIENTS WITH ADVANCED PROSTATE CARCINOMA - A NEW THERAPEUTIC APPROACH [J].
BORGMANN, V ;
HARDT, W ;
SCHMIDTGOLLWITZER, M ;
ADENAUER, H ;
NAGEL, R .
LANCET, 1982, 1 (8281) :1097-1099
[5]  
Bosland M C, 2000, J Natl Cancer Inst Monogr, P39
[6]  
BOSLAND MC, 1992, J CELL BIOCHEM, P89
[7]   Structure and properties of carotenoids in relation to function [J].
Britton, G .
FASEB JOURNAL, 1995, 9 (15) :1551-1558
[8]   EVALUATION OF 10 PAIRWISE MULTIPLE COMPARISON PROCEDURES BY MONTE-CARLO METHODS [J].
CARMER, SG ;
SWANSON, MR .
JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION, 1973, 68 (341) :66-74
[9]   Mass spectrometric determination of genistein tissue distribution in diet-exposed Sprague-Dawley rats [J].
Chang, HC ;
Churchwell, MI ;
Delclos, KB ;
Newbold, RR ;
Doerge, DR .
JOURNAL OF NUTRITION, 2000, 130 (08) :1963-1970
[10]   Diet, nutrition, and prostate cancer [J].
Clinton, SK ;
Giovannucci, E .
ANNUAL REVIEW OF NUTRITION, 1998, 18 :413-440