Involvement of protein dynamics in enzyme stability -: The case of glucose oxidase

被引：22

作者：

Haouz, A ^{[1
]}

Glandiéres, JM ^{[1
]}

Alpert, B ^{[1
]}

机构：

[1] Univ Denis Diderot, Lab Biol Physicochim, F-75251 Paris 05, France

来源：

FEBS LETTERS | 2001年 / 506卷 / 03期

关键词：

protein dynamics; glucose oxidase; immobilized protein; thermostability; fluorescence quenching;

D O I：

10.1016/S0014-5793(01)02917-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynamics of glucose oxidase immobilized and in solution were compared through their tryptophan fluorescence spectra, decay times and quenching by acrylamide. Energy barrier for thermal inactivation and melting temperature of both soluble and immobilized enzyme were also measured. Data show that the fluctuation amplitude is at the origin of protein instability. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：216 / 220

页数：5

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