Solid-State NMR Characterization of Gas Vesicle Structure

被引：36

作者：

Sivertsen, Astrid C. ^{[1
,2
]}

Bayro, Marvin J. ^{[1
,2
,3
]}

Belenky, Marina ^{[1
]}

Griffin, Robert G. ^{[2
,3
]}

Herzfeld, Judith ^{[1
]}

机构：

[1] Brandeis Univ, Dept Chem, Waltham, MA 02254 USA

[2] MIT, Francis Bitter Magnet Lab, Cambridge, MA 02139 USA

[3] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

BIOPHYSICAL JOURNAL | 2010年 / 99卷 / 06期

基金：

美国国家卫生研究院;

关键词：

ANABAENA-FLOS-AQUAE; NUCLEAR-MAGNETIC-RESONANCE; ALPHA-SYNUCLEIN FIBRILS; AIR-WATER-INTERFACE; BLUE-GREEN ALGA; CHEMICAL-SHIFTS; CORRELATION SPECTROSCOPY; CROSS-POLARIZATION; ROTATING SOLIDS; SECONDARY STRUCTURE;

D O I：

10.1016/j.bpj.2010.06.041

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-alpha-beta-beta-alpha-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.

引用

页码：1932 / 1939

页数：8

共 57 条

[1] Probing through-bond connectivities and through-space distances in solids by magic-angle-spinning nuclear magnetic resonance [J].

Baldus, M ;

Iuliucci, RJ ;

Meier, BH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (05) :1121-1124

[2]

Baldus M, 1998, MOL PHYS, V95, P1197, DOI 10.1080/00268979809483251

[3] Total correlation spectroscopy in the solid state. The use of scalar couplings to determine the through-bond connectivity [J].

Baldus, M ;

Meier, BH .

JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 121 (01) :65-69

[4] Radio frequency-driven recoupling at high magic-angle spinning frequencies: Homonuclear recoupling sans heteronuclear decoupling [J].

Bayro, Marvin J. ;

Ramachandran, Ramesh ;

Caporini, Marc A. ;

Eddy, Matthew T. ;

Griffin, Robert G. .

JOURNAL OF CHEMICAL PHYSICS, 2008, 128 (05)

[5] Long-Range Correlations between Aliphatic 13C Nuclei in Protein MAS NMR Spectroscopy [J].

Bayro, Marvin J. ;

Maly, Thorsten ;

Birkett, Neil R. ;

Dobson, Christopher M. ;

Griffin, Robert G. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (31) :5708-5710

[6] Subunit structure of gas vesicles: A MALDI-TOF mass spectrometry study [J].

Belenky, M ;

Meyers, R ;

Herzfeld, J .

BIOPHYSICAL JOURNAL, 2004, 86 (01) :499-505

[7] HETERONUCLEAR DECOUPLING IN ROTATING SOLIDS [J].

BENNETT, AE ;

RIENSTRA, CM ;

AUGER, M ;

LAKSHMI, KV ;

GRIFFIN, RG .

JOURNAL OF CHEMICAL PHYSICS, 1995, 103 (16) :6951-6958

[8] Homonuclear radio frequency-driven recoupling in rotating solids [J].

Bennett, AE ;

Rienstra, CM ;

Griffiths, JM ;

Zhen, WG ;

Lansbury, PT ;

Griffin, RG .

JOURNAL OF CHEMICAL PHYSICS, 1998, 108 (22) :9463-9479

[9] CHEMICAL-SHIFT CORRELATION SPECTROSCOPY IN ROTATING SOLIDS - RADIO FREQUENCY-DRIVEN DIPOLAR RECOUPLING AND LONGITUDINAL EXCHANGE [J].

BENNETT, AE ;

OK, JH ;

GRIFFIN, RG ;

VEGA, S .

JOURNAL OF CHEMICAL PHYSICS, 1992, 96 (11) :8624-8627

[10] CRYSTALLINE-STRUCTURE OF GAS VESICLE WALL FROM ANABAENA-FLOS-AQUAE [J].

BLAUROCK, AE ;

WALSBY, AE .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 105 (02) :183-199

← 1 2 3 4 5 6 →