Application of reproductive technology to buffaloes

This paper focuses on recent advances in the application of embryo transfer technologies to buffalo. The early recovery rate of 0.15 transferable embryos has now increased to 2.0, with altered superovulation protocols. Lower follicular population and poor follicular development, with adverse seasonal influences explain the lower ovulatory responses. Unovulated follicles at superovulation contribute high quantities of oestrogen, altering the uterine milieu. The birth of calves using in vitro fertilisation technology and frozen-thawed embryos and determination of embryonic sex using a Y-specific DNA probe, are recent milestones achieved using these technologies.