Droplet microfluidics: recent developments and future applications

被引:288
作者
Casadevall i Solvas, Xavier [1 ]
deMello, Andrew [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
基金
英国工程与自然科学研究理事会;
关键词
PROTEIN EXPRESSION; CELL; MONODISPERSE; SYSTEMS; EMULSION; ASSAYS; SCALE; DNA; MICRODROPLETS; ENCAPSULATION;
D O I
10.1039/c0cc02474k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We report recent advances in the field of droplet-based microfluidics. Specifically, we highlight the unique features of such platforms for high-throughput experimentation; describe functional components that afford complex analytical processing and report on applications in synthesis, high-throughput screening, cell biology and synthetic and systems biology. Issues including the integration of high-information content detection methods, long term droplet stability and opportunities for large scale and intelligent biological experimentation are also discussed.
引用
收藏
页码:1936 / 1942
页数:7
相关论文
共 85 条
[1]   The Digital Revolution: A New Paradigm for Microfluidics [J].
Abdelgawad, Mohamed ;
Wheeler, Aaron R. .
ADVANCED MATERIALS, 2009, 21 (08) :920-925
[2]   Formation of dispersions using "flow focusing" in microchannels [J].
Anna, SL ;
Bontoux, N ;
Stone, HA .
APPLIED PHYSICS LETTERS, 2003, 82 (03) :364-366
[3]   Quantitative Cell-Based Reporter Gene Assays Using Droplet-Based Microfluidics [J].
Baret, Jean-Christophe ;
Beck, Yannick ;
Billas-Massobrio, Isabelle ;
Moras, Dino ;
Griffiths, Andrew D. .
CHEMISTRY & BIOLOGY, 2010, 17 (05) :528-536
[4]   Droplet interface bilayers [J].
Bayley, Hagan ;
Cronin, Brid ;
Heron, Andrew ;
Holden, Matthew A. ;
Hwang, William L. ;
Syeda, Ruhma ;
Thompson, James ;
Wallace, Mark .
MOLECULAR BIOSYSTEMS, 2008, 4 (12) :1191-1208
[5]   Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in C. elegans [J].
Ben-Yakar, Adela ;
Chronis, Nikos ;
Lu, Hang .
CURRENT OPINION IN NEUROBIOLOGY, 2009, 19 (05) :561-567
[6]   Simple, robust storage of drops and fluids in a microfluidic device [J].
Boukellal, Hakim ;
Selimovic, Seila ;
Jia, Yanwei ;
Cristobal, Galder ;
Fraden, Seth .
LAB ON A CHIP, 2009, 9 (02) :331-338
[7]   Microfluidics-based systems biology [J].
Breslauer, DN ;
Lee, PJ ;
Lee, LP .
MOLECULAR BIOSYSTEMS, 2006, 2 (02) :97-112
[8]   Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets [J].
Bringer, MR ;
Gerdts, CJ ;
Song, H ;
Tice, JD ;
Ismagilov, RF .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 2004, 362 (1818) :1087-1104
[9]   Droplet microfluidic technology for single-cell high-throughput screening [J].
Brouzes, Eric ;
Medkova, Martina ;
Savenelli, Neal ;
Marran, Dave ;
Twardowski, Mariusz ;
Hutchison, J. Brian ;
Rothberg, Jonathan M. ;
Link, Darren R. ;
Perrimon, Norbert ;
Samuels, Michael L. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) :14195-14200
[10]   Droplet-based microfluidics for emulsion and solvent evaporation synthesis of monodisperse mesoporous silica microspheres [J].
Carroll, Nick J. ;
Rathod, Shailendra B. ;
Derbins, Erin ;
Mendez, Sergio ;
Weitz, David A. ;
Petsev, Dimiter N. .
LANGMUIR, 2008, 24 (03) :658-661