Quantification of in vivo fluorescence decoupled from the effects of tissue optical properties using fiber-optic spectroscopy measurements

被引：138

作者：

Kim, Anthony ^{[2
]}

Khurana, Mamta ^{[2
]}

Moriyama, Yumi ^{[1
]}

Wilson, Brian C. ^{[1
,2
]}

机构：

[1] Univ Hlth Network, Ontario Canc Inst, Campbell Family Inst Canc Res, Toronto, ON M5G 2M9, Canada

[2] Univ Toronto, Dept Med Biophys, Toronto, ON M5G 2M9, Canada

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 06期

基金：

加拿大自然科学与工程研究理事会; 美国国家卫生研究院;

关键词：

fluorescence spectroscopy; turbid media; diffuse reflectance; cancer diagnosis; fluorescence quantification; tissue optics; protoporphyrin IX; PHOTODYNAMIC THERAPY; TURBID MEDIA; FLUOROPHORE CONCENTRATION; GLIOBLASTOMA-MULTIFORME; INTRINSIC FLUORESCENCE; 5-AMINOLEVULINIC ACID; LIGHT-PROPAGATION; GUIDED RESECTION; SCATTERING; PORPHYRINS;

D O I：

10.1117/1.3523616

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast. (C) 2010 society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3523616]

引用

页数：12

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