Improvements in the sensitivity of time resolved fluorescence energy transfer assays

被引：19

作者：

Jones, SG ^{[1
]}

Lee, DY ^{[1
]}

Wright, JF ^{[1
]}

Jones, CN ^{[1
]}

Teear, ML ^{[1
]}

Gregory, SJ ^{[1
]}

Burns, DD ^{[1
]}

机构：

[1] Nycomed Amersham Plc, Cardiff Labs, Forest Farm, Cardiff CF14 7YT, S Wales, Wales

来源：

JOURNAL OF FLUORESCENCE | 2001年 / 11卷 / 01期

关键词：

fluorescence resonance energy transfer; time resolved fluorescence; multiple labelling; p65; binding; helicase;

D O I：

10.1023/A:1016691414432

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect on fluorescence resonance energy transfer (FRET) of multiple labelling of DNA oligonucleotides with donor lanthanide chelate and acceptor CyDye(TM) fluors has been investigated. It is shown that using a multiple donor lanthanide chelate with a single acceptor Cy(TM)3 or Cy5 can increase sensitivity and fluorescence output. The enhanced FRET observed in the multiple donor label system has been utilised in two different DNA based assay formats to demonstrate the advantages over a steady state fluorescence assay and a radiometric assay.

引用

页码：13 / 21

页数：9

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