Endosomal clathrin drives actin accumulation at the immunological synapse

被引：62

作者：

Calabia-Linares, Carmen ^{[1
]}

Robles-Valero, Javier ^{[1
]}

de la Fuente, Hortensia ^{[1
]}

Perez-Martinez, Manuel ^{[1
]}

Martin-Cofreces, Noa ^{[1
,2
]}

Alfonso-Perez, Manuel ^{[1
]}

Gutierrez-Vazquez, Cristina ^{[2
]}

Mittelbrunn, Maria ^{[2
]}

Ibiza, Sales ^{[2
]}

Urbano-Olmos, Francisco R. ^{[3
]}

Aguado-Ballano, Covadonga ^{[3
]}

Oscar Sanchez-Sorzano, Carlos ^{[4
]}

Sanchez-Madrid, Francisco ^{[1
]}

Veiga, Esteban ^{[1
]}

机构：

[1] Hosp Univ Princesa, Serv Immunol, Inst Invest Sanitaria Hosp Princesa IP, Madrid 28006, Spain

[2] Ctr Nacl Invest Cardiovasc, Dept Biol Vasc & Inflamac, Madrid 28029, Spain

[3] Univ Autonoma Madrid, Fac Med, Lab Microscopia Elect Transmis, E-28029 Madrid, Spain

[4] CSIC, Unidad Biocomputac, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

来源：

JOURNAL OF CELL SCIENCE | 2011年 / 124卷 / 05期

关键词：

Actin; Clathrin; Cytoskeleton; Immunological synapse; T-CELL-ACTIVATION; HUNTINGTIN-INTERACTING PROTEIN-1; ENDOCYTIC MACHINERY; IMMUNE SYNAPSE; COATED PITS; MEDIATED ENDOCYTOSIS; HIP1-RELATED PROTEIN; MAMMALIAN-CELLS; HIP1R; CYTOSKELETON;

D O I：

10.1242/jcs.078832

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antigen-specific cognate interaction of T lymphocytes with antigen-presenting cells (APCs) drives major morphological and functional changes in T cells, including actin rearrangements at the immune synapse (IS) formed at the cell-cell contact area. Here we show, using cell lines as well as primary cells, that clathrin, a protein involved in endocytic processes, drives actin accumulation at the IS. Clathrin is recruited towards the IS with parallel kinetics to that of actin. Knockdown of clathrin prevents accumulation of actin and proteins involved in actin polymerization, such as dynamin-2, the Arp2/3 complex and CD2AP at the IS. The clathrin pool involved in actin accumulation at the IS is linked to multivesicular bodies that polarize to the cell-cell contact zone, but not to plasma membrane or Golgi complex. These data underscore the role of clathrin as a platform for the recruitment of proteins that promote actin polymerization at the interface of T cells and APCs.

引用

页码：820 / 830

页数：11

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