Rapid screening procedures for identification of succinic acid producers

被引：39

作者：

Agarwal, L ^{[1
]}

Isar, J ^{[1
]}

Saxena, RK ^{[1
]}

机构：

[1] Univ Delhi, Dept Microbiol, New Delhi 110021, India

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2005年 / 63卷 / 01期

关键词：

succinic acid; anaerobic organisms; TLC; fluorescent method;

D O I：

10.1016/j.jbbm.2005.01.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Succinic acid, an intermediate of tricarboxylic acid cycle. is produced and accumulated by anaerobic microorganisms. The long-standing interest in the production of this organic acid is because it is a key compound in producing more than 30 commercially important products. The detection of succinic acid is generally carried out by gas chromatography (GC), enzymatic assays, ion-exclusion chromatography (IEC) or by high performance liquid chromatography (HPLC). However, these methods are time consuming, require sophisticated instrumentation and are expensive. In the present investigation we are reporting two rapid, cost effective screening methods for the detection of this important organic acid. These methods can be utilized to screen a large number of microbes producing succinic acid in a very short span of time. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：24 / 32

页数：9

共 24 条

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