The impact of next-generation sequencing on genomics

被引:250
作者
Zhang, Jun [1 ,2 ]
Chiodini, Rod
Badr, Ahmed [1 ]
Zhang, Genfa [3 ]
机构
[1] Texas Tech Univ, Hlth Sci Ctr, Dept Anesthesiol, COE Neurosci, El Paso, TX 79905 USA
[2] Texas Tech Univ, Hlth Sci Ctr, Dept Biomed Sci, El Paso, TX 79905 USA
[3] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
关键词
Next-generation sequencing; Genomics; Genetic variation; Polymorphism; Targeted sequence enrichment; Bioinformatics; HIGH-THROUGHPUT; CHALLENGES; MUTATIONS; ALIGNMENT; CAPTURE; OLIGONUCLEOTIDES; POLYMORPHISMS; TECHNOLOGIES; PREDICTION; READS;
D O I
10.1016/j.jgg.2011.02.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This article reviews basic concepts, general applications, and the potential impact of next-generation sequencing (NGS) technologies on genomics, with particular reference to currently available and possible future platforms and bioinformatics. NGS technologies have demonstrated the capacity to sequence DNA at unprecedented speed, thereby enabling previously unimaginable scientific achievements and novel biological applications. But, the massive data produced by NGS also presents a significant challenge for data storage, analyses, and management solutions. Advanced bioinformatic tools are essential for the successful application of NGS technology. As evidenced throughout this review, NGS technologies will have a striking impact on genomic research and the entire biological field. With its ability to tackle the unsolved challenges unconquered by previous genomic technologies, NGS is likely to unravel the complexity of the human genome in terms of genetic variations, some of which may be confined to susceptible loci for some common human conditions. The impact of NGS technologies on genomics will be far reaching and likely change the field for years to come.
引用
收藏
页码:95 / 109
页数:15
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