Phospholipase A2-like catalytic antibody

被引：7

作者：

Isomura, S ^{[1
]}

Ito, K ^{[1
]}

Haruna, M ^{[1
]}

机构：

[1] Meijo Univ, Fac Pharm, Tempaku Ku, Nagoya, Aichi 468, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1999年 / 9卷 / 04期

关键词：

D O I：

10.1016/S0960-894X(99)00035-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The phospholipase A(2)-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C-2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a k(cat) of 1.0 x 10(-2) min(-1) and a K-m of 71 mu M. This antibody hydrolyzes the C-2 ester in (R)-2, regio- and enantioselectively. (C) 1999 Elsevier Science Ltd. Ail rights reserved.

引用

页码：555 / 558

页数：4

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