Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol

被引：22

作者：

Wang, Ying ^{[1
,2
]}

Zhang, Di ^{[1
]}

He, Fu Rong ^{[3
]}

Chen, Xiao Chun ^{[3
]}

机构：

[1] Beijing Normal Univ, Sch Environm, Key Lab Water & Sediment Sci, Minist Educ, Beijing 100875, Peoples R China

[2] Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China

[3] Beijing Inst Chem Technol, Dept Chem Engn, Beijing 100029, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2012年 / 23卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Magnetic silica; Immobilization; Laccase; Pentachlorophenol; BEADS; CHLOROPHENOLS; DEGRADATION; CATALASE;

D O I：

10.1016/j.cclet.2011.10.011

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Magnetic Cu2+-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination. The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol (PCP) degradation were evaluated systemically. Compared with free laccase, the immobilized laccase showed improved acid adaptability and thermal stability. The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions. (C) 2011 Ying Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

引用

页码：197 / 200

页数：4

共 8 条

[1] A new metal-chelated beads for reversible use in uricase adsorption [J].

Akgoel, Sinan ;

Oeztuerk, Nevra ;

Karagoezler, A. Alev ;

Uygun, Deniz Aktas ;

Uygun, Murat ;

Denizli, Adil .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2008, 51 (1-2) :36-41

[2] Preparation of Cu(II) adsorbed chitosan beads for catalase immobilization [J].

Cetinus, Senay Akkus ;

Sahin, Ebru ;

Saraydin, Dursun .

FOOD CHEMISTRY, 2009, 114 (03) :962-969

[3] Sequential photochemical-biological degradation of chlorophenols [J].

Essam, Tamer ;

Amin, Magdy Aly ;

El Tayeb, Ossama ;

Mattiasson, Bo ;

Guieysse, Benoit .

CHEMOSPHERE, 2007, 66 (11) :2201-2209

[4] Immobilization of Pycnoporus sanguineus laccase on magnetic chitosan microspheres [J].

Jiang, DS ;

Long, SY ;

Huang, J ;

Xiao, HY ;

Zhou, JY .

BIOCHEMICAL ENGINEERING JOURNAL, 2005, 25 (01) :15-23

[5] Laccase immobilization on mesostructured cellular foams affords preparations with ultra high activity [J].

Rekuc, Adriana ;

Bryjak, Jolanta ;

Szymanska, Katarzyna ;

Jarzebski, Andrzej B. .

PROCESS BIOCHEMISTRY, 2009, 44 (02) :191-198

[6] Reversible immobilization of catalase by metal chelate affinity interaction on magnetic beads [J].

Sari, M ;

Akgol, S ;

Karatas, M ;

Denizli, A .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2006, 45 (09) :3036-3043

[7] Degradation of chlorophenols catalyzed by laccase [J].

Zhang, Jianbo ;

Liu, Xiaopeng ;

Xu, Zhenqiang ;

Chen, Hui ;

Yang, Yuxiang .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2008, 61 (04) :351-356

[8] Removal of 2,4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor [J].

Zhang, Jianbo ;

Xu, Zhenqiang ;

Chen, Hui ;

Zong, Yueru .

BIOCHEMICAL ENGINEERING JOURNAL, 2009, 45 (01) :54-59

← 1 →