Separation of nucleotides using micellar electrokinetic capillary chromatography

The effect of the pH of the background electrolyte and its ionic strength, via Good's buffers, in capillary zone electrophoresis (CZE) on the separation of mono-, di-, triphosphate, adenosine cyclic-monophosphate nucleosides and adenine was investigated. Micellar electrokinetic capillary chromatography (MEKCC) using a cationic surfactant such as dodecyltrimethylammonium bromide offers better separation and shorter analysis than the sodium dodecyl sulfate micellar system. Extension of the elution range could be performed by decreasing the pH and efficiency was improved by adding ethylenediaminetetraacetic acid to the mixture sample reaching 130000 and 60000 theoretical plates for c-AMP and GDP, respectively. (C) 1998 Elsevier Science B.V.