Competitive binding assay for thyroxine using in vitro selected oligonucleotides

被引：14

作者：

Ito, Y ^{[1
]}

Fujita, S

Kawazoe, N

Imanishi, Y

机构：

[1] NAIST, Grad Sch Mat Sci, Ikoma 6300101, Japan

[2] JST, PRESTO, Kyoto 6190237, Japan

[3] Kyoto Univ, Grad Sch Engn, Dept Med Chem, Kyoto 6068501, Japan

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 16期

关键词：

D O I：

10.1021/ac9800816

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new binding assay that uses oligodeoxyribonucleotides (DNAs) obtained by the in vitro selection method, instead of antibodies, to bind to the target molecule, thyroxine (T-4), is described, The DNAs which selectively bound to the T-4 were selected, labeled with biotin or radioisotope, and then used to detection of T-4 in the presence of liothyronine (T-3), which has a chemical structure similar to that of T-4.

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收藏

页码：3510 / 3512

页数：3

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