The catalysed NADH reduction of resazurin to resorufin

被引：54

作者：

Candeias, LP

MacFarlane, DPS

McWhinnie, SLW

Maidwell, NL

Roeschlaub, CA

Sammes, PG ^{[1
]}

Whittlesey, R

机构：

[1] Univ Surrey, Sch Phys Sci, Dept Chem, Mol Probes Unit, Guildford GU2 5XH, Surrey, England

[2] Brunel Univ, Dept Chem, Uxbridge UB8 3PH, Middx, England

[3] Mt Vernon Hosp, Gray Lab Canc Res Trust, Northwood HA6 2JR, Middx, England

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 | 1998年 / 11期

关键词：

D O I：

10.1039/a806431h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Details are reported on the mechanism whereby NADH can be used for the reduction of resazurin 1 to give the fluorescent product resorufin 2, a process requiring the use of a catalyst, such as N-methylphenazinium methosulfate 3.

引用

页码：2333 / 2334

页数：2

共 17 条

[1]

BLANKENHORN G, 1977, PYRIDINE NUCLEOTIDE, P185

[2]

COOK DB, 1993, CLIN CHEM, V39, P965

[3] FREE-RADICAL PRODUCTION FROM THE AEROBIC OXIDATION OF REDUCED PYRIDINE-NUCLEOTIDES CATALYZED BY PHENAZINE DERIVATIVES [J].

DAVIS, G ;

THORNALLEY, PJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 724 (03) :456-464

[4]

HALAKA FG, 1982, J BIOL CHEM, V257, P1458

[5]

HAUGHLAND RP, 1997, HDB FLUORESCENT PROB, P381

[6]

IMAMURA H, 1994, CANCER RES, V54, P3620

[7] On the acknowledgement of the phenazine [J].

Kehrmann, F ;

Havas, E .

BERICHTE DER DEUTSCHEN CHEMISCHEN GESELLSCHAFT, 1913, 46 :341-352

[8] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[9] OCCURRENCE OF SUPEROXIDE ANION IN REACTION OF REDUCED PHENAZINE METHOSULFATE AND MOLECULAR-OXYGEN [J].

NISHIKIMI, M ;

APPAJI, N ;

YAGI, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 46 (02) :849-+

[10] STUDIES ON REDUCTION OF NITROBLUE TETRAZOLIUM CHLORIDE MEDIATED THROUGH ACTION OF NADH AND PHENAZINE METHOSULFATE [J].

PONTI, V ;

DIANZANI, MU ;

CHEESEMAN, K ;

SLATER, TF .

CHEMICO-BIOLOGICAL INTERACTIONS, 1978, 23 (03) :281-291

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