Characterization of two Sp1 binding sites of the human sex determining SRY promoter

被引：27

作者：

Desclozeaux, M ^{[1
]}

Poulat, F ^{[1
]}

Barbara, PD ^{[1
]}

Soullier, S ^{[1
]}

Jay, P ^{[1
]}

Berta, P ^{[1
]}

Boizet-Bonhoure, B ^{[1
]}

机构：

[1] CNRS, CRBM, ERS155, F-34293 Montpellier 5, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 03期

关键词：

SRY gene; binding site; promoter; (human);

D O I：

10.1016/S0167-4781(98)00041-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the molecular basis of the human SRY gene regulation, we have examined the significance of two potential binding sites for the transcription factor Sp1 (Sp1A: -124 to -131 and Sp1B: -147 to -154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in Drosophila SL2 cells implicated Spl protein in the transcriptional activation of the SRY promoter. (C) 1998 Elsevier Science B.V.

引用

页码：247 / 252

页数：6

共 31 条

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