Picomole scale stereochemical analysis of sphingosines and dihydrosphingosines

被引：32

作者：

Kawamura, A

Berova, N

Dirsch, V

Mangoni, A

Nakanishi, K

Schwartz, G

Bielawska, A

Hannun, Y

Kitagawa, I

机构：

[1] COLUMBIA UNIV,DEPT CHEM,NEW YORK,NY 10027

[2] MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021

[3] DUKE UNIV,MED CTR,DEPT MED & CELL BIOL,DURHAM,NC 27710

[4] OSAKA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,SUITA,OSAKA 565,JAPAN

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1996年 / 4卷 / 07期

关键词：

D O I：

10.1016/0968-0896(96)00092-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple picomole (low nanogram) scale HPLC scheme which can separate all eight isomers of sphingosine and dihydrosphingosine thus leading to the identification of their relative and absolute configurations. The amino group of the sample is derivatized to its fluorescent N-naphthimide which is analyzed by normal and chiral phase HPLC, coupled with fluorescence peak detection. If necessary, the results of this HPLC method can be further corroborated by measurements of circular dichroic (CD) spectra of the N-naphthimido-derivatives and/or N,O-chromophoric derivatives. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：1035 / 1043

页数：9

共 26 条

[1]

BUEHRER BM, 1992, J BIOL CHEM, V267, P3154

[2]

CARTER HE, 1947, J BIOL CHEM, V170, P285

[3]

CARTER HE, 1951, J BIOL CHEM, V191, P727

[4] SYNTHESIS OF ERYTHRO-ALPHA-BETA-HYDROXYSTEARIC ACID AND THREO-ALPHA-AMINO-BETA-HYDROXYSTEARIC ACIDS [J].