Molecular and biochemical characterization of a novel class A β-lactamase (HER-1) from Escherichia hermannii

被引：13

作者：

Beauchef-Havard, A

Arlet, G

Gautier, V

Labia, R

Grimont, P

Philippon, A

机构：

[1] Hop Tenon, Serv Bacteriol, AP HP, F-75670 Paris 20, France

[2] UFR Saint Antoine, Paris, France

[3] Inst Pasteur, INSERM, U389, Unite Enterobacteries, F-75724 Paris, France

[4] CNRS, UBO, MHN, Unite FRE 2125, Quimper, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 08期

关键词：

D O I：

10.1128/AAC.47.8.2669-2673.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Escherichia hermannii showed a low level of resistance to amoxicillin and ticarcillin, reversed by clavulanate, and a moderate susceptibility to piperacillin but was susceptible to all cephalosporins. A bla gene was cloned and encoded a typical class A beta-lactamase (HER-1, pI 7.5), which shares 45, 449 41, and 40% amino acid identity, with other beta-lactamases, AER-1 from Aeromonas hydrophila, MAL-1/Cko-1 from Citrobacter koseri, and TEM-1 and LEN-1, respectively. No ampR gene was detected. Only penicillins were efficiently hydrolyzed, and no hydrolysis was observed for cefuroxime and broad-spectrum cephalosporins. Sequencing of the bla gene in 12 other strains showed 98 to 100% identity with bla(HER-1).

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页码：2669 / 2673

页数：5

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