Biological consequences of the heterogeneous irradiation of lymphocytes during technetium-99m hexamethylpropylene amine oxime white blood cell labelling

被引:24
作者
de Labriolle-Vaylet, C
Colas-Linhart, N
Sala-Trepat, M
Petiet, A
Voisin, P
Bok, B
机构
[1] Univ Paris 06, Serv Biophys, Paris, France
[2] Fac Xavier Bichat, Biophys Lab, Paris, France
[3] Inst Curie, CNRS, URA 1292, F-75231 Paris, France
[4] IPSN, Lab Dosimetrie Biol Multiparametr, Fontenay Aux Roses, France
关键词
technetium-99m hexamethylpropylene amine oxime; microautoradiography; heterogeneous labelling; lymphocyte viability; irradiation effects;
D O I
10.1007/s002590050318
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Technetium-99m hexamethylpropylene amine oxime ((99m)Tc-HMPAO) labelling of white blood cells, routinely used for the detection of infection, results in the incorporation of radioactivity by polymorphonuclear leucocytes and also lymphocytes and can induce cell lesions in the latter case. The aim of this study was therefore to acquire data on the morphological and functional status of labelled lymphocytes present in the (99m)Tc-HMPAO leucocyte mixture and to determine the cellular consequences of labelling. The mean radioactivity associated with lymphocytes was 325+/-10.8 kBq/10(6) lymphocytes under standard labelling conditions. Microautoradiographic studies showed that labelling was heterogeneous (4% intensely labelled cells), which prevented calculation of the mean absorbed dose. The frequency of chromosomal aberrations (dicentrics and rings) in the labelled lymphocytes for 380 kBq/10(6) cells was 1.08+/-0.09 but no abnormality was observed in the unlabelled control lymphocytes. The plating efficiency of labelled lymphocytes was reduced, as compared with that for control cells, but some lymphocytes were still able to form clones and were still "alive" by radiobiological definition. It is therefore suggested that lymphocytes should be removed from (99m)Tc-HMPAO cell preparations before administration to patients.
引用
收藏
页码:1423 / 1428
页数:6
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