共 55 条
Site-Specific Identification of SUMO-2 Targets in Cells Reveals an Inverted SUMOylation Motif and a Hydrophobic Cluster SUMOylation Motif
被引:253
作者:

Matic, Ivan
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

论文数: 引用数:
h-index:
机构:

Hendriks, Ivo A.
论文数: 0 引用数: 0
h-index: 0
机构:
Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

van Santen, Maria A.
论文数: 0 引用数: 0
h-index: 0
机构:
Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

van de Rijke, Frans
论文数: 0 引用数: 0
h-index: 0
机构:
Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

论文数: 引用数:
h-index:
机构:

Gnad, Florian
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Vertegaal, Alfred C. O.
论文数: 0 引用数: 0
h-index: 0
机构:
Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
机构:
[1] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
[2] Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands
关键词:
IN-VIVO IDENTIFICATION;
MASS-SPECTROMETRY;
QUANTITATIVE PROTEOMICS;
PROTEIN SUMOYLATION;
PROXIMITY LIGATION;
UBIQUITIN;
TRANSCRIPTION;
CONJUGATION;
COMPLEXES;
RANGAP1;
D O I:
10.1016/j.molcel.2010.07.026
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Reversible protein modification by small ubiquitin-like modifiers (SUMOs) is critical for eukaryotic life. Mass spectrometry-based proteomics has proven effective at identifying hundreds of potential SUMO target proteins. However, direct identification of SUMO acceptor lysines in complex samples by mass spectrometry is still very challenging. We have developed a generic method for the identification of SUMO acceptor lysines in target proteins. We have identified 103 SUMO-2 acceptor lysines in endogenous target proteins. Of these acceptor lysines, 76 are situated in the SUMOylation consensus site [VILMFPC]KxE. Interestingly, eight sites. fit the inverted SUMOylation consensus motif [ED]xK[VILFP]. In addition, we found direct mass spectrometric evidence for crosstalk between SUMOylation and phosphorylation with a preferred spacer between the SUMOylated lysine and the phosphorylated serine of four residues. In 16 proteins we identified a hydrophobic cluster SUMOylation motif (HCSM). SUMO conjugation of RanGAP1 and ZBTB1 via HCSMs is remarkably efficient.
引用
收藏
页码:641 / 652
页数:12
相关论文
共 55 条
[1]
Principles of ubiquitin and SUMO modifications in DNA repair
[J].
Bergink, Steven
;
Jentsch, Stefan
.
NATURE,
2009, 458 (7237)
:461-467

Bergink, Steven
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany

Jentsch, Stefan
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany
[2]
Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1
[J].
Bernier-Villamor, V
;
Sampson, DA
;
Matunis, MJ
;
Lima, CD
.
CELL,
2002, 108 (03)
:345-356

Bernier-Villamor, V
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Sampson, DA
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Matunis, MJ
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA

Lima, CD
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Biochem, Struct Biol Program, New York, NY 10021 USA
[3]
In Vivo Identification of Sumoylation Sites by a Signature Tag and Cysteine-targeted Affinity Purification
[J].
Blomster, Henri A.
;
Imanishi, Susumu Y.
;
Siimes, Jenny
;
Kastu, Juha
;
Morrice, Nick A.
;
Eriksson, John E.
;
Sistonen, Lea
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2010, 285 (25)
:19324-19329

Blomster, Henri A.
论文数: 0 引用数: 0
h-index: 0
机构:
Abo Akad Univ, Turku Ctr Biotechnol, FI-20521 Turku, Finland
Univ Turku, FI-20521 Turku, Finland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland

Imanishi, Susumu Y.
论文数: 0 引用数: 0
h-index: 0
机构:
Abo Akad Univ, Turku Ctr Biotechnol, FI-20521 Turku, Finland
Univ Turku, FI-20521 Turku, Finland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland

论文数: 引用数:
h-index:
机构:

Kastu, Juha
论文数: 0 引用数: 0
h-index: 0
机构:
Abo Akad Univ, Turku Ctr Biotechnol, FI-20521 Turku, Finland
Univ Turku, FI-20521 Turku, Finland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland

Morrice, Nick A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Dundee, Coll Life Sci, MRC, Prot Phosphorylat Unit, Dundee DD1 5EH, Scotland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland

Eriksson, John E.
论文数: 0 引用数: 0
h-index: 0
机构:
Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland
Abo Akad Univ, Turku Ctr Biotechnol, FI-20521 Turku, Finland
Univ Turku, FI-20521 Turku, Finland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland

Sistonen, Lea
论文数: 0 引用数: 0
h-index: 0
机构:
Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland
Abo Akad Univ, Turku Ctr Biotechnol, FI-20521 Turku, Finland
Univ Turku, FI-20521 Turku, Finland Abo Akad Univ, Dept Biosci, FI-20520 Turku, Finland
[4]
Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions
[J].
Choudhary, Chunaram
;
Kumar, Chanchal
;
Gnad, Florian
;
Nielsen, Michael L.
;
Rehman, Michael
;
Walther, Tobias C.
;
Olsen, Jesper V.
;
Mann, Matthias
.
SCIENCE,
2009, 325 (5942)
:834-840

Choudhary, Chunaram
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany
Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Prot Res, DK-2200 Copenhagen, Denmark Max Planck Inst Biochem, D-82152 Martinsried, Germany

Kumar, Chanchal
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Gnad, Florian
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Nielsen, Michael L.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany
Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Prot Res, DK-2200 Copenhagen, Denmark Max Planck Inst Biochem, D-82152 Martinsried, Germany

Rehman, Michael
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Walther, Tobias C.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Olsen, Jesper V.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany
Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Prot Res, DK-2200 Copenhagen, Denmark Max Planck Inst Biochem, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany
Univ Copenhagen, Fac Hlth Sci, Novo Nordisk Fdn Ctr Prot Res, DK-2200 Copenhagen, Denmark Max Planck Inst Biochem, D-82152 Martinsried, Germany
[5]
Is proteomics the new genomics?
[J].
Cox, Juergen
;
Mann, Matthias
.
CELL,
2007, 130 (03)
:395-398

Cox, Juergen
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany
[6]
A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics
[J].
Cox, Juergen
;
Matic, Ivan
;
Hilger, Maximiliane
;
Nagaraj, Nagarjuna
;
Selbach, Matthias
;
Olsen, Jesper V.
;
Mann, Matthias
.
NATURE PROTOCOLS,
2009, 4 (05)
:698-705

Cox, Juergen
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Matic, Ivan
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Hilger, Maximiliane
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Nagaraj, Nagarjuna
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Selbach, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Delbruck Ctr Mol Med, Dept Cell Signalling & Mass Spectrometry, Berlin, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Olsen, Jesper V.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
[7]
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
[J].
Cox, Juergen
;
Mann, Matthias
.
NATURE BIOTECHNOLOGY,
2008, 26 (12)
:1367-1372

Cox, Juergen
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
[8]
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
[J].
Daub, Henrik
;
Olsen, Jesper V.
;
Bairlein, Michaela
;
Gnad, Florian
;
Oppermann, Felix S.
;
Koerner, Roman
;
Greff, Zoltan
;
Keri, Gyoergy
;
Stemmann, Olaf
;
Mann, Matthias
.
MOLECULAR CELL,
2008, 31 (03)
:438-448

Daub, Henrik
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Olsen, Jesper V.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Bairlein, Michaela
论文数: 0 引用数: 0
h-index: 0
机构: Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Gnad, Florian
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Oppermann, Felix S.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Koerner, Roman
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Greff, Zoltan
论文数: 0 引用数: 0
h-index: 0
机构:
Vichem Chem Ltd, H-1022 Budapest, Hungary Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Keri, Gyoergy
论文数: 0 引用数: 0
h-index: 0
机构:
Vichem Chem Ltd, H-1022 Budapest, Hungary
Semmelweis Univ, Hungarian Acad Sci, Pathobiochem Res Grp, H-1088 Budapest, Hungary Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Stemmann, Olaf
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Mol Cell Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Biol, Cell Signaling Grp, D-82152 Martinsried, Germany
[9]
Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
[J].
de Godoy, Lyris M. F.
;
Olsen, Jesper V.
;
Cox, Juergen
;
Nielsen, Michael L.
;
Hubner, Nina C.
;
Froehlich, Florian
;
Walther, Tobias C.
;
Mann, Matthias
.
NATURE,
2008, 455 (7217)
:1251-U60

de Godoy, Lyris M. F.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Olsen, Jesper V.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Cox, Juergen
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Nielsen, Michael L.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Hubner, Nina C.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Froehlich, Florian
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Walther, Tobias C.
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany

Mann, Matthias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem, D-82152 Martinsried, Germany
[10]
A proteomic strategy for gaining insights into protein sumoylation in yeast
[J].
Denison, C
;
Rudner, AD
;
Gerber, SA
;
Bakalarski, CE
;
Moazed, D
;
Gygi, SP
.
MOLECULAR & CELLULAR PROTEOMICS,
2005, 4 (03)
:246-254

Denison, C
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Rudner, AD
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Gerber, SA
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Bakalarski, CE
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Moazed, D
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

Gygi, SP
论文数: 0 引用数: 0
h-index: 0
机构: Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA