Inhibition of PhIP mutagenicity by caffeine, lycopene, daidzein, and genistein

被引:45
作者
Weisburger, JH [1 ]
Dolan, L [1 ]
Pittman, B [1 ]
机构
[1] Amer Hlth Fdn, Valhalla, NY 10595 USA
关键词
phytochemicals; tea; caffeine; lycopene; daidzein; genistein; 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine PhIP; mutagenicity; antioxidant; inhibition;
D O I
10.1016/S1383-5718(98)00083-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a major dietary component in individuals eating cooked meats or fish. This heterocyclic amine requires biochemical activation, mainly through cytochrome P4501A2, and can be detoxified chiefly by 4'-hydroxylation through other cytochromes, and be in turn converted through phase 2 enzymes to readily excreted conjugates. The active form of PhIP is mutagenic in Salmonella typhimurium TA98 and is a useful substrate to study the possible chemoprotective action of phytochemicals. We found that black and green tea depressed the mutagenicity of PhIP in dose-related fashion, and decaffeinated tea was less powerful an inhibitor. This led to the study of caffeine, that displayed effective dose-related inhibition of the mutagenicity of PhIP. Other antioxidants such as lycopene, the active antioxidant from tomatoes, and daidzein and genistein from soy products, also had a dose-related inhibition of the mutagenicity of PhIP. We conclude that PhIP is a good substrate found in several human foods to determine the protective effect of phytochemicals from vegetables, fruits and beverages. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:125 / 128
页数:4
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