Enhanced oligonucleotide binding to self-assembled nanofibers

被引:43
作者
Guler, MO
Pokorski, JK
Appella, DH
Stupp, SI
机构
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[2] Northwestern Univ, Dept Mat Sci & Engn, Evanston, IL 60208 USA
[3] Northwestern Univ, Feinberg Sch Med, Evanston, IL 60208 USA
关键词
D O I
10.1021/bc050053b
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A peptide nucleic acid/peptide amphiphile conjugate (PNA-PA) that self-assembles into fiber-shaped nanostructures was designed to bind oligonucleotides with high affinity and specificity. Oligonucleotide binding to PNA-PA nanofibers was studied using fluorescence polarization, and thermal stability was examined by UV-vis measurement of duplex melting temperatures. The self-assembled PNA-PA DNA system was observed to bind more strongly than the corresponding DNA-DNA duplex. We also observed single base specificity with a 16 degrees C in thermal stability. As expected from the previous PNA studies, PNA-PA RNA binding is also stronger than the corresponding RNA-RNA duplex.
引用
收藏
页码:501 / 503
页数:3
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