Detection of Fasciola hepatica antigen in cattle faeces by a monoclonal antibody-based sandwich immunoassay

被引：27

作者：

Dumenigo, BE

Espino, AM

Finlay, CM

机构：

[1] Subdirección de Parasitol., Inst. Med. Trop. 'Pedro Kouri', Ciudad de La Habana, Apartado 601

来源：

RESEARCH IN VETERINARY SCIENCE | 1996年 / 60卷 / 03期

关键词：

D O I：

10.1016/S0034-5288(96)90055-7

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A monoclonal antibody-based sandwich immunoassay (mAb sandwich ELISA) was developed for the detection of Fasciola hepatica antigen in the faeces of cattle. The assay was applied to samples from 100 cattle infected with F hepatica, 56 animals with parasitologically proven infections of other parasites and 100 uninfected animals. F hepatica antigen was detected in all the faecal samples from animals with fasciolosis, but none of the samples from the uninfected animals or from those with other parasitic infections had significant levels of F hepatica antigens. The results indicate that the mAb sandwich ELISA is a rapid, simple and useful method for the diagnosis of active F hepatica infection in cattle.

引用

页码：278 / 279

页数：2

共 11 条

[1] THE PREPATENT AND PATENT PERIODS OF FASCIOLA-HEPATICA IN CATTLE IN PUERTO-RICO [J].