A single amino acid change converts an inhibitory transcription factor into an activator

被引：29

作者：

Dawson, SJ ^{[1
]}

Morris, PJ ^{[1
]}

Latchman, DS ^{[1
]}

机构：

[1] UCL, SCH MED, MED MOLEC BIOL UNIT, DEPT MOLEC PATHOL, LONDON W1P 6DB, ENGLAND

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 20期

关键词：

D O I：

10.1074/jbc.271.20.11631

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The closely related POU family transcription factors Brn-3a and Brn-3b differ in their functional activity with Brn-3a activating several target promoters, which are repressed by Brn-3b. Brn-3b also prevents promoter activation by Brn-3a. Here we have altered a single isoleucine residue in the POU homeodomain of Brn-3b to the valine residue found at the equivalent position in Brn-3a. This change not only abolishes the ability of Brn-3b to repress basal and Brn-3a-stimulated promoter activity but also converts it to an activator of similar potency to Brn-3a. Hence a single amino acid difference determines the difference between an activator and a repressor in the Brn-3 family.

引用

页码：11631 / 11633

页数：3

共 27 条

[1] A PROCEDURE TO STANDARDIZE CAT REPORTER GENE ASSAY
ABKEN, H
REIFENRATH, B
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (13) : 3527 - 3527
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site
BudhramMahadeo, V
Morris, PJ
Lakin, ND
Dawson, SJ
Latchman, DS
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (15) : 9108 - 9113
[4] ACTIVATION OF THE ALPHA-INTERNEXIN PROMOTER BY THE BRN-3A TRANSCRIPTION FACTOR IS DEPENDENT ON THE N-TERMINAL REGION OF THE PROTEIN
BUDHRAMMAHADEO, V
MORRIS, PJ
LAKIN, ND
THEIL, T
CHING, GY
LILLYCROP, KA
MOROY, T
LIEM, RKH
LATCHMAN, DS
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (06) : 2853 - 2858
[5] THE DNA TARGET SITE FOR THE BRN-3 POU FAMILY TRANSCRIPTION FACTORS CAN CONFER RESPONSIVENESS TO CYCLIC-AMP AND REMOVAL OF SERUM IN NEURONAL CELLS
BUDHRAMMAHADEO, V
THEIL, T
MORRIS, PJ
LILLYCROP, KA
MOROY, T
LATCHMAN, DS
[J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (15) : 3092 - 3098
[6] The ability of POU family transcription factors to activate or repress gene expression is dependent on the spacing and context of their specific response elements
Dawson, SJ
Liu, YZ
Rodel, B
Moroy, T
Latchman, DS
[J]. BIOCHEMICAL JOURNAL, 1996, 314 : 439 - 443
[7] A GENETIC PATHWAY FOR THE DEVELOPMENT OF THE CAENORHABDITIS-ELEGANS HSN MOTOR NEURONS
DESAI, C
GARRIGA, G
MCINTIRE, SL
HORVITZ, HR
[J]. NATURE, 1988, 336 (6200) : 638 - 646
[8] BRN-3.0 EXPRESSION IDENTIFIES EARLY POSTMITOTIC CNS NEURONS AND SENSORY NEURAL PRECURSORS
FEDTSOVA, NG
TURNER, EE
[J]. MECHANISMS OF DEVELOPMENT, 1995, 53 (03) : 291 - 304
[9] THE C-ELEGANS CELL LINEAGE AND DIFFERENTIATION GENE UNC-86 ENCODES A PROTEIN WITH A HOMEODOMAIN AND EXTENDED SIMILARITY TO TRANSCRIPTION FACTORS
FINNEY, M
RUVKUN, G
HORVITZ, HR
[J]. CELL, 1988, 55 (05) : 757 - 769
[10] BRN-3.0 - A POU-DOMAIN PROTEIN EXPRESSED IN THE SENSORY, IMMUNE, AND ENDOCRINE SYSTEMS THAT FUNCTIONS ON ELEMENTS DISTINCT FROM KNOWN OCTAMER MOTIFS
GERRERO, MR
MCEVILLY, RJ
TURNER, E
LIN, CR
OCONNELL, S
JENNE, KJ
HOBBS, MV
ROSENFELD, MG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (22) : 10841 - 10845

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