Applications to cancer research of "lab-on-a-chip" devices based on dielectrophoresis (DEP)

被引:42
作者
Gambari, R
Borgatti, M
Altomare, L
Manaresi, N
Medoro, G
Romani, A
Tartagni, M
Guerrieri, R
机构
[1] Univ Ferrara, Ctr Biotechnol, I-44100 Ferrara, Italy
[2] Silicon Biosyst, I-40125 Bologna, Italy
[3] Univ Bologna, Ctr Elect Elect Syst, ARCES, I-40125 Bologna, Italy
[4] Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy
关键词
dielectrophoresis; lab-on-a-chip; cell manipulation;
D O I
10.1177/153303460300200105
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The recent development of advanced analytical and bioseparation methodologies based on microarrays and biosensors is one of the strategic objectives of the so-called post-genomic. In this field, the development of microfabricated devices could bring new opportunities in several application fields, such as predictive oncology, diagnostics and anti-tumor drug research. The so called "Laboratory-on-a-chip technology", involving miniaturisation of analytical procedures, is expected to enable highly complex laboratory testing to move from the central laboratory into non-laboratory settings. The main advantages of Lab-on-a-chip devices are integration of multiple steps of different analytical procedures, large variety of applications, sub-microliter consumption of reagents and samples, and portability. One of the requirement for new generation Lab-on-a-chip devices is the possibility to be independent from additional preparative/analytical instruments. Ideally, Lab-on-a-chip devices should be able to perform with high efficiency and reproducibility both actuating and sensing procedures. In this review, we discuss applications of dielectrophoretic(DEP)-based Lab-on-a-chip devices to cancer research. The theory of dielectrophoresis as well as the description of several devices, based on spiral-shaped, parallel and arrayed electrodes are here presented. In addition, in this review we describe manipulation of cancer cells using advanced DEP-based Lab-on-a-chip devices in the absence of fluid flow and with the integration of both actuating and sensing procedures.
引用
收藏
页码:31 / 39
页数:9
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