The first example of an RNA urea synthase: Selection through the enzyme active site of human neutrophile elastase

被引：15

作者：

Nieuwlandt, D ^{[1
]}

West, M ^{[1
]}

Cheng, XQ ^{[1
]}

Kirshenheuter, G ^{[1
]}

Eaton, BE ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Chem, Coll Phys & Math Sci, Raleigh, NC 27695 USA

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 07期

关键词：

diastereoselectivity; enzyme catalysis; peptides; RNA; stereoselection;

D O I：

10.1002/cbic.200300610

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA catalysts: In vitro selection from a random library of RNA sequences gave RNA urea synthases able to utilize tripeptides as substrates (see scheme; HNE, human neutrophile elastase). The tripeptide substrates were mechanism-based protease suicide substrates. Stereoselection of serine protease tripeptide phosphonate inhibitors was achieved by RNA catalysis. The RNA urea synthases showed a high degree of diastereoselectivity for the most potent protease suicide inhibitor.

引用

页码：651 / 654

页数：4

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