Interleukin-1β Induces Angiogenesis and Innervation in Human Intervertebral Disc Degeneration

被引：184

作者：

Lee, Jae Man ^{[2
]}

Song, Ji Ye ^{[3
]}

Baek, MinJung ^{[3
]}

Jung, Hye-Young ^{[4
]}

Kang, Haeyoun ^{[5
]}

Han, In Bo ^{[6
]}

Do Kwon, Young ^{[1
]}

Shin, Dong Eun ^{[2
]}

机构：

[1] CHA Univ, Dept Biomed Sci, Grad Sch, Gyeongg Do 487010, South Korea

[2] CHA Univ, Dept Orthopaed Surg, CHA Bundang Med Ctr, Gyeonggi Do 463712, South Korea

[3] CHA Univ, Inst Clin Res, CHA Bundang Med Ctr, Gyeonggi Do 463712, South Korea

[4] CHA Univ, Dept Med Educ, CHA Bundang Med Ctr, Gyeonggi Do 463712, South Korea

[5] CHA Univ, Dept Pathol, CHA Bundang Med Ctr, Gyeonggi Do 463712, South Korea

[6] CHA Univ, Dept Neurosurg, CHA Bundang Med Ctr, Gyeonggi Do 463712, South Korea

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2011年 / 29卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

interleukin-1; beta; vascular endothelial growth factor (VEGF); angiogenesis; innervation; intervertebral disc (IVD); NECROSIS-FACTOR-ALPHA; DEGRADATION; EXPRESSION; TISSUE; PAIN; TNF;

D O I：

10.1002/jor.21210

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Degenerative disorders of the intervertebral discs (IVDs) are generally characterized by enhanced matrix degradation, angiogenesis, innervation, and increased expression of catabolic cytokines. In this study, we investigated the effects of inflammatory cytokines, IL-1 beta, and TNF-alpha, on the expression of an angiogenic factor, vascular endothelial growth factor (VEGF), and neurotrophic factors, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), in human IVD degeneration. IL-1 beta and TNF-alpha stimulated the gene expression of VEGF, NGF, and BDNF in nucleus pulposus (NP) cells isolated from patient tissues. Immunohistochemical results demonstrated a positive correlation between IL-1 beta and VEGF/NGF/BDNF expression in human IVD tissues. RNA expression analysis of patient tissues also identified positive correlations between VEGF and platelet endothelial cell adhesion molecule-1 (PECAM-1) and between NGF/BDNF and protein gene product 9.5 (PGP9.5). Our findings suggest that IL-1 beta is generated during IVD degeneration, which stimulates the expression of VEGF, NGF, and BDNF, resulting in angiogenesis and innervation. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J. Orthop. Res. 29: 265-269, 2011

引用

页码：265 / 269

页数：5

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