Improvement of PCR for detection of Opisthorchis vivenini DNA in human stool samples

被引：56

作者：

Duenngai, Kunyarat ^{[1
,2
]}

Sithithaworn, Paiboon ^{[1
,2
]}

Rudrappa, Urnesha Kanasinakatte ^{[3
]}

Iddya, Karunasagar ^{[3
]}

Laha, Thewarach ^{[1
]}

Stensvold, Christen Rune ^{[4
]}

Strandgaard, Hanne ^{[4
]}

Johansen, Maria Vang ^{[4
]}

机构：

[1] Khon Kaen Univ, Fac Med, Dept Parasitol, Khon Kaen 40002, Thailand

[2] Khon Kaen Univ, Fac Med, LFCRC, Khon Kaen 40002, Thailand

[3] Univ Agr Sci Mangalore, Coll Fisheries, Dept Fishery Microbiol, Mangalore 575002, India

[4] DBL Ctr Hlth Res & Dev, DK-2920 Charlottenlund, Denmark

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 01期

关键词：

D O I：

10.1128/JCM.01323-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction protocol for the detection of O. viverrini DNA using cetyltrimethyl-ammoniumbromide to remove PCR inhibitor was used and compared with the commercial stool kit method. The sensitivity of the new test was 79.3%, compared with the 44.8% of the previous method (P < 0.01). PCR-positive tests identified several cases judged parasite negative by the parasitological method (28.6%), indicating the new test's advantage in the diagnosis of individuals with light infections.

引用

页码：366 / 368

页数：3

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