A stress-, pathogenesis-, and allergen-related cDNA in apple fruit is also ripening-related

被引:46
作者
Atkinson, RG [1 ]
Perry, J [1 ]
Matsui, T [1 ]
Ross, GS [1 ]
Macrae, EA [1 ]
机构
[1] KAGAWA UNIV,FAC AGR,DEPT AGROIND SCI,MIKI,KAGAWA 76107,JAPAN
关键词
allergen; apple; pathogenesis-related; ripening; stress;
D O I
10.1080/01140671.1996.9513941
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The screening of a ripe apple (Malus domestica 'Golden Delicious') cDNA expression library with polyclonal antibodies to spinach sucrose-phosphate synthase resulted in the isolation of a cDNA clone pAP15. This clone encodes a homologue of a class of stress and pathogenesis-related clones, and is also highly homologous to a class of allergenic proteins from apple without known biological function. The pAP15 cDNA insert is 817 base pairs in length and codes fbr a protein of 17.5 kD. Genomic Southern analysis suggests that the gene belongs to a family of at least 15 members. Northern analysis indicates that pAP15 is ripening-related, with an accumulation of homologous mRNA coincident with ethylene production during apple fruit ripening.
引用
收藏
页码:103 / 107
页数:5
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