Identification of Rickettsia felis in the salivary glands of cat fleas

被引:36
作者
Macaluso, Kevin R. [1 ]
Pornwiroon, Walairat [1 ]
Popov, Vsevolod L. [2 ]
Foil, Lane D. [3 ]
机构
[1] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA
[2] Univ Texas Galveston, Dept Pathol, WHO Collaborating Ctr Trop Dis, Med Branch, Galveston, TX USA
[3] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70803 USA
关键词
cat flea; Ctenocephalides felis; Rickettsia felis;
D O I
10.1089/vbz.2007.0218
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Rickettsia felis, a flea-associated rickettsial pathogen, has been identified in many tissues, including the digestive and reproductive tissues, within the cat flea, Ctenocephalides felis. We utilized transmission electron microscopy and polymerase chain reaction to identify R. felis in the salivary glands of fed fleas and further define the distribution of R. felis within the arthropod host. We identified Rickettsia-like organisms in salivary glands using electron microscopy. Sequence analysis of portions of the Rickettsia genus-specific 17-kDa antigen gene and R. felis plasmid confirmed the morphological identification of R. felis in cat flea salivary glands. This is the first report of R. felis in tissues critical for horizontal transmissions of rickettsiae.
引用
收藏
页码:391 / 396
页数:6
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