Germ-line transgenesis of the Tc1/mariner superfamily transposon Minos in Ciona intestinalis

被引：101

作者：

Sasakura, Y ^{[1
]}

Awazu, S ^{[1
]}

Chiba, S ^{[1
]}

Satoh, N ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Zool, Sakyo Ku, Kyoto 6068502, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 13期

关键词：

ascidian; transgenic technique; enhancer trap;

D O I：

10.1073/pnas.1230736100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The tadpole larva of the basal chordate Ciona intestinalis has the most simplified, basic body-plan of chordates. Because it has a compact genome with a complete draft sequence, a large quantity of EST/cDNA information, and a short generation time, Ciona is a suitable model for future genetics. We establish here a transgenic technique in Ciona that uses the Tc1/mariner superfamily transposon Minos. Minos was integrated efficiently into the genome of germ cells and transmitted stably to subsequent generations. In addition, an enhancer-trap line was obtained. This is a demonstration of efficient, Minos-mediated transgenesis in marine invertebrates.

引用

页码：7726 / 7730

页数：5

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