Purinergic and adrenergic Ca2+ transients during neurogenic contractions of rat mesenteric small arteries

被引:50
作者
Lamont, C [1 ]
Vainorius, E [1 ]
Wier, WG [1 ]
机构
[1] Univ Maryland, Dept Physiol, Sch Med, Baltimore, MD 21201 USA
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2003年 / 549卷 / 03期
关键词
D O I
10.1113/jphysiol.2003.043380
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Contraction of small arteries is regulated by the sympathetic nervous system, but the Ca2+ transients during neurally stimulated contraction of intact small arteries have not yet been recorded. We loaded rat mesenteric small arteries with the fluorescent Ca2+ indicator fluo-4 and mounted them in a myograph that permitted simultaneous (i) high-speed confocal imaging of fluorescence from individual smooth muscle cells, (ii) electrical stimulation of perivascular nerves, and (iii) recording of isometric tension. Sympathetic neuromuscular transmission was achieved by electrical field stimulation (EFS) (frequency, 10 Hz; pulse voltage, 40 V; pulse duration, 0.2 ms) in the presence of capsaicin and scopolamine (to inhibit 'sensory' and cholinergic nerves, respectively). During the first 20 s of EFS, force rose to a small peak and then declined. During this time, junctional Ca2+ transients (jCaTs) were present at relatively high frequency. We have previously attributed jCaTs to influx of Ca2+ through post-junctional P2X receptors activated by ATP. Propagating asynchronous Ca2+ waves, previously associated with bath-applied alpha(1)-adrenoceptor agonists, were not initially present. During the next 2.5 min of EFS, force rose slowly, and asynchronous propagating Ca2+ waves appeared. The selective alpha(1)-adrenoceptor antagonist prazosin abolished both the slowly developing contraction and the Ca2+ waves, but reduced the initial transient contraction by only similar to25 %. During 3 min of EFS in prazosin, the frequency of jCaTs declined markedly; at sites at which at least one jCaT occurred, the average probability of a jCaT was 0.008 +/- 0.002 pulse(-1) in the first 20 s and 0.0007 +/- 0.0002 pulse(-1) in the last 20 s. We suggest that (i) ATP released from sympathetic varicosities activates the initial, transient, contraction and the activator Ca 21 is derived largely from jCaTs, and (ii) sympathetically released noradrenaline (NA) activates the later, major contraction through mechanisms involving alpha(1)-adrenoceptors and which are associated with propagating Ca2+ waves.
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页码:801 / 808
页数:8
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