New Developments in Pichia pastoris Expression System, Review and Update

被引:46
作者
Baghban, Roghayyeh [1 ,2 ,3 ]
Farajnia, Safar [3 ,4 ]
Ghasemi, Younes [5 ]
Mortazavi, Mojtaba [6 ]
Zarghami, Nosratollah [1 ]
Samadi, Naser [1 ]
机构
[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Med Biotechnol Dept, Tabriz, Iran
[2] Tabriz Univ Med Sci, Res Comm, Tabriz, Iran
[3] Tabriz Univ Med Sci, Biotechnol Res Ctr, Daneshgah Ave, Tabriz, Iran
[4] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
[5] Shiraz Univ Med Sci, Fac Pharm & Pharmaceut Sci, Res Ctr, Dept Pharmaceut Biotechnol, Shiraz, Iran
[6] Grad Univ Adv Technol, Inst Sci & High Technol & Environm Sci, Dept Biotechnol, Kerman, Iran
关键词
Pichia pastoris; expression system; recombinant protein; glycoengineering; Crispr/Cas9; genome engineering; RECOMBINANT PROTEIN-PRODUCTION; HISTAMINE H-1 RECEPTOR; HIGH-LEVEL EXPRESSION; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; HETEROLOGOUS PROTEINS; COPY NUMBER; FUNCTIONAL-CHARACTERIZATION; MONOCLONAL-ANTIBODY; ALCOHOL OXIDASE;
D O I
10.2174/1389201019666180718093037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Background: Although Pichia pastoris is an outstanding host among conventional expression systems for production of recombinant proteins, a new interest has been emerged to this system due to the inherent advantages and new developments in this expression host. The potential for secretory and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate for the production of complex eukaryotic proteins. Methods: Several new developments have occurred in different areas related to P. pastoris expression system including hosts, vectors, glycosylation pattern and fermentation technology. Strain engineering using Crispr/Cas9 technology to produce human-like glycoproteins and protease deficient strains are two new areas of development with high importance. Results: This review is dedicated to discuss the most important characteristics of P. pastoris with emphasis on new developments, especially in the field of glycoengineering, efficient expression vectors and promoters. Conclusion: New developments that occurred in the P. pastoris expression system converted this system to a versatile host for the production of complex proteins. This progress paved the way for several proteins to enter the clinical trials or industrial processes with this valuable expression host.
引用
收藏
页码:451 / 467
页数:17
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