Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)

被引:107
作者
Chua, Lee Suan [1 ]
Latiff, Norliza Abdul [1 ]
Lee, Sze Yean [1 ]
Lee, Chew Tin [2 ]
Sarmidi, Mohamad Roji [1 ]
Aziz, Ramlan Abdul [1 ]
机构
[1] Univ Teknol Malaysia, Metabolites Profiling Lab, Utm Skudai 81310, Johor, Malaysia
[2] Univ Teknol Malaysia, Fac Chem Engn, Dept Bioproc Engn, Utm Skudai 81310, Johor, Malaysia
关键词
Labisia pumila; Flavonols; Flavanols; Phenolic acids; UPLC-ESI-MS/MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; DISCRIMINANT-ANALYSIS; EXTRACTS; COMPONENTS; WHITE; HERB;
D O I
10.1016/j.foodchem.2011.01.122
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The 40% MeOH fraction exhibited the highest scavenging activity. Nine flavonols (quercetin, myricetin and kaempferol), two flavanols (catechin and epigallocatechin) and nine phenolic acids were identified from this active fraction by UPLC-ESI-MS/MS, and confirmed by comparison with the mass spectra of standard aglycones, theoretical fragments generated from MS Fragmenter software, and literature values. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1186 / 1192
页数:7
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