Interaction of syntenin-1 and the NG2 proteoglycan in migratory oligodendrocyte precursor cells

被引：50

作者：

Chatterjee, Nivedita ^{[1
]}

Stegmueller, Judith ^{[1
]}

Schaetzle, Philipp ^{[1
]}

Karram, Khalad ^{[1
]}

Koroll, Michael ^{[2
]}

Werner, Hauke B. ^{[3
]}

Nave, Klaus-Armin ^{[3
]}

Trotter, Jacqueline ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Dept Biol, D-55128 Mainz, Germany

[2] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany

[3] Max Planck Inst Expt Med, Dept Neurogenet, D-37075 Gottingen, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2008年 / 283卷 / 13期

关键词：

D O I：

10.1074/jbc.M706074200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Migration of oligodendrocyte precursors along axons is a necessary prerequisite for myelination, but little is known about underlying mechanisms. NG2 is a large membrane proteoglycan implicated in oligodendrocyte migration. Here we show that a PDZ domain protein termed syntenin-1 interacts with NG2 and that syntenin-1 is necessary for normal rates of migration. The association of syntenin-1 with NG2, identified in a yeast two-hybrid screen, was confirmed by colocalization of both proteins within processes of oligodendroglial precursor cells and by coimmunoprecipitation from cell extracts. Syntenin-1 also colocalizes with NG2 in "co-capping" assays, demonstrating a lateral association of both proteins in live oligodendrocytes. RNA interference-mediated down-regulation of syntenin-1 in glial cells results in a significant reduction of migration in vitro, as does the presence of polyclonal antibody against NG2. Thus syntenin plays a role in the migration of oligodendroglial precursors, and we suggest that NG2-syntenin-1 interactions contribute to this.

引用

页码：8310 / 8317

页数：8

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