Human homologs of the Xenopus oocyte cortical granule lectin XL35

被引:88
作者
Lee, JK
Schnee, J
Pang, M
Wolfert, M
Baum, LG
Moremen, KW
Pierce, M
机构
[1] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
[2] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
[3] Univ Calif Los Angeles, Sch Med, Dept Pathol, Div Cardiol, Los Angeles, CA 90024 USA
[4] Univ Calif Los Angeles, Sch Med, Dept Med, Div Cardiol, Los Angeles, CA 90024 USA
关键词
lectin; endothelial cells;
D O I
10.1093/glycob/11.1.65
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cDNAs encoding two human homologs of the Xenopus,us oocyte lectin, XL35, were isolated from a small intestine cDNA library and termed HL-1 and HL-2, The deduced amino acid sequence of each homolog is about 60% identical and 80% similar to that of XL35, and none of these sequences contains the C-type lectin motif, although it is known that XL35 requires calcium for ligand binding. By Northern analysis, HL-1 transcripts are present at relatively high levels in heart, small intestine, colon, thymus, ovary, and testis, HL-2 transcripts, by contrast, are expressed only in small intestine. Immuraocytochemistry using a polyclonal antibody produced against XL35 shows HL-1 protein to be localized exclusively in endothelial cells in colon, thymus, liver, and other tissues. Primary cultures of human aortic endothelial cells are positive for HL-1 expression by immunoblotting and by PCR analysis, but several other human cell types are not. HL-1 and -2 are both encoded at chromosome 1q23, the same locus that encodes the selectins, XL35, HL-1 and -2, and another mouse homolog are members of a new family of proteins whose members most likely perform diverse functions.
引用
收藏
页码:65 / 73
页数:9
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