Field-evolved resistance to Cry1Ab maize by Spodoptera frugiperda in Brazil

被引:232
作者
Omoto, Celso [1 ]
Bernardi, Oderlei [1 ]
Salmeron, Eloisa [1 ]
Sorgatto, Rodrigo J. [1 ]
Dourado, Patrick M. [2 ]
Crivellari, Augusto [2 ]
Carvalho, Renato A. [2 ]
Willse, Alan [3 ]
Martinelli, Samuel [3 ]
Head, Graham P. [3 ]
机构
[1] Univ Sao Paulo, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13418900 Sao Paulo, Brazil
[2] Monsanto Brazil Ltda, Sao Paulo, Brazil
[3] Monsanto Co, St Louis, MO USA
关键词
fall armyworm; Cry1Ab protein; Bt maize; insect resistance management; FALL ARMYWORM LEPIDOPTERA; BASE-LINE SUSCEPTIBILITY; BACILLUS-THURINGIENSIS; BT-MAIZE; NOCTUIDAE POPULATIONS; TRANSGENIC CROPS; DELTA-ENDOTOXIN; PUERTO-RICO; TOXIN; CORN;
D O I
10.1002/ps.4201
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
BACKGROUNDThe first Bt maize in Brazil was launched in 2008 and contained the MON 810 event, which expresses Cry1Ab protein. Although the Cry1Ab dose in MON 810 is not high against fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), MON 810 provided commercial levels of control. To support insect resistance management in Brazil, the baseline and ongoing susceptibility of FAW was examined using protein bioassays, and the level of control and life history parameters of FAW were evaluated on MON 810 maize. RESULTSBaseline diet overlay assays with Cry1Ab (16 mu g cm(-2)) caused 76.3% mortality to field FAW populations sampled in 2009. Moderate mortality (48.8%) and significant growth inhibition (88.4%) were verified in leaf-disc bioassays. In greenhouse trials, MON 810 had significantly less damage than non-Bt maize. The surviving FAW larvae on MON 810 (22.4%) had a 5.5 day increase in life cycle time and a 24% reduction in population growth rate. Resistance monitoring (2010-2015) showed a significant reduction in Cry1Ab susceptibility of FAW over time. Additionally, a significant reduction in the field efficacy of MON 810 maize against FAW was observed in different regions from crop season 2009 to 2013. CONCLUSIONSThe decrease in susceptibility to Cry1Ab was expected, but the specific contributions to this resistance by MON 810 maize cannot be distinguished from cross-resistance to Cry1Ab caused by exposure to Cry1F maize. Technologies combining multiple novel insecticidal traits with no cross-resistance to the current Cry1 proteins and high activity against the same target pests should be pursued in Brazil and similar environments. (c) 2015 Society of Chemical Industry
引用
收藏
页码:1727 / 1736
页数:10
相关论文
共 61 条
[1]  
[Anonymous], 2014, AC SAFR BRAS GRAOS
[2]   Interactions of Bacillus thuringiensis crystal proteins with the midgut epithelial cells of Spodoptera frugiperda (Lepidoptera: Noctuidae) [J].
Aranda, E ;
Sanchez, J ;
Peferoen, M ;
Guereca, L ;
Bravo, A .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1996, 68 (03) :203-212
[3]   Oviposition, Development, and Reproduction of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) Fed on Different Hosts of Economic Importance [J].
Barros, Eduardo M. ;
Torres, Jorge B. ;
Bueno, Adeney F. .
NEOTROPICAL ENTOMOLOGY, 2010, 39 (06) :996-1001
[4]   Cross-Resistance between Cry1 Proteins in Fall Armyworm (Spodoptera frugiperda) May Affect the Durability of Current Pyramided Bt Maize Hybrids in Brazil [J].
Bernardi, Daniel ;
Salmeron, Eloisa ;
Horikoshi, Renato Jun ;
Bernardi, Oderlei ;
Dourado, Patrick Marques ;
Carvalho, Renato Assis ;
Martinelli, Samuel ;
Head, Graham P. ;
Omoto, Celso .
PLOS ONE, 2015, 10 (10)
[5]   Frequency of resistance to Vip3Aa20 toxin from Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in Brazil [J].
Bernardi, Oderlei ;
Bernardi, Daniel ;
Ribeiro, Rebeca S. ;
Okuma, Daniela M. ;
Salmeron, Eloisa ;
Fatoretto, Julio ;
Medeiros, Fernanda C. L. ;
Burd, Tony ;
Omoto, Celso .
CROP PROTECTION, 2015, 76 :7-14
[6]   Compared biology of Spodoptera frugiperda (J.E.!Smith) (Lepidoptera: Noctuidae) populations in corn and rice leaves [J].
Busato, GR ;
Grützmacher, AD ;
Garcia, MS ;
Giolo, FP ;
Zotti, MJ ;
Stefanello, J .
NEOTROPICAL ENTOMOLOGY, 2005, 34 (05) :743-750
[7]   Peer-reviewed surveys indicate positive impact of commercialized GM crops [J].
Carpenter, Janet E. .
NATURE BIOTECHNOLOGY, 2010, 28 (04) :319-321
[8]   Investigating the Molecular Mechanisms of Organophosphate and Pyrethroid Resistance in the Fall Armyworm Spodoptera frugiperda [J].
Carvalho, Renato A. ;
Omoto, Celso ;
Field, Linda M. ;
Williamson, Martin S. ;
Bass, Chris .
PLOS ONE, 2013, 8 (04)
[9]  
Celeres, 2014, IB1403 CEL
[10]   YIELD IMPACT OF LARVAL INFESTATIONS OF THE FALL ARMYWORM (LEPIDOPTERA, NOCTUIDAE) TO MIDWHORL GROWTH STAGE OF CORN [J].
CRUZ, I ;
TURPIN, FT .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1983, 76 (05) :1052-1054