Lack of the DNA repair protein O-6-methylguanine-DNA methyltransferase in histologically normal brain adjacent to primary human brain tumors

被引:68
作者
Silber, JR
Blank, A
Bobola, MS
Mueller, BA
Kolstoe, DD
Ojemann, GA
Berger, MS
机构
[1] UNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195
[2] UNIV WASHINGTON,DEPT EPIDEMIOL,SEATTLE,WA 98195
[3] UNIV WASHINGTON,CHILDRENS HOSP & MED CTR,DEPT SURG,DIV NEUROL SURG,SEATTLE,WA 98105
[4] FRED HUTCHINSON CANC RES CTR,DIV PUBL HLTH,SEATTLE,WA 98104
关键词
aging; alkylation; carcinogenesis; DNA damage; mutagenesis;
D O I
10.1073/pnas.93.14.6941
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Exposure to exogenous alkylating agents, particularly N-nitroso compounds, has been associated with increased incidence of primary human brain tumors, while intrinsic risk factors are currently unknown. The DNA repair protein O-6-methylguanine-DNA methyltransferase (MGMT) is a major defense against the carcinogenicity of N-nitroso compounds and other alkylators. We report here that in 55% (64/117) of cases, histologically normal brain tissue adjacent to primary human brain tumors lacked detectable MGMT activity [methyl excision repair-defective (Mer(-)) status]. The incidence of Mer(-) status in normal brain tissue from brain tumor patients was age-dependent, increasing from 21% in children 0.25-19 years of age to 75% in adults over 50. In contrast, Mer(-) status was found in 12% (5/43) of normal brain specimens from patients operated for conditions other than primary brain tumors and was not age-dependent. The 4.6-fold elevation in incidence of Mer(-) status in brain tumor patients is highly significant (chi(2) = 24; p less than or equal to 0.001). MGMT activity was independent of age in the lymphocytes of brain tumor patients and was present in lymphocytes from six of nine tumor patients whose normal brain specimen was Mer(-). DNA polymerase beta, apurinic/apyrimidinic endonuclease, and lactate dehydrogenase activities were present in all specimens tested, including Mer(-) specimens from brain tumor patients. Our data are consistent with a model of carcinogenesis in human brain in which epigenetically regulated lack of MGMT is a predisposing factor and alkylation-related mutagenesis is a driving force.
引用
收藏
页码:6941 / 6946
页数:6
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