A novel selection system for potato transformation using a mutated AHAS gene

被引：41

作者：

Andersson, M

Trifonova, A

Andersson, AB

Johansson, M

Bülow, L

Hofvander, P

机构：

[1] Plant Sci Sweden AB, S-26831 Svalov, Sweden

[2] Lund Univ, S-22100 Lund, Sweden

[3] Swedish Univ Agr Sci, Dept Plant Biol, S-75007 Uppsala, Sweden

来源：

PLANT CELL REPORTS | 2003年 / 22卷 / 04期

关键词：

acetohydroxyacid synthase; AHAS; herbicide tolerance; selection gene; potato transformation;

D O I：

10.1007/s00299-003-0684-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a beta-glucuronidase (GUS) gene. Selection on 0.5 muM Imazamox yielded GUS expression in 93-100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

引用

页码：261 / 267

页数：7

共 32 条

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