Oral immunisation with live aroA attenuated Salmonella enterica serovar Typhimurium expressing the Yersinia pestis V antigen protects mice against plague

被引：43

作者：

Garmory, HS ^{[1
]}

Griffin, KF

Brown, KA

Titball, RW

机构：

[1] Porton Down, Dstl Chem & Biol Sci, Salisbury SP4 0JQ, Wilts, England

[2] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, Dept Biol Sci, London SW7 2AY, England

[3] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England

来源：

VACCINE | 2003年 / 21卷 / 21-22期

关键词：

plague; V antigen; Salmonella enterica serovar Typhimurium;

D O I：

10.1016/S0264-410X(03)00112-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Bubonic and pneumonic plague are caused by the bacterium Yersinia pestis. The V antigen of Y. pestis is a protective antigen against plague. In this study, an aroA attenuated strain of Salmonella enterica serovar Typhimurium (SL3261) has been used to deliver the Y. pestis V antigen as a candidate oral plague vaccine. SL3261 was transformed with the expression plasmid pTrc-LcrV, containing the lcrV gene encoding V antigen. Immunoblot analysis showed V antigen expression in SL3261 in vitro and intragastric immunisation of mice with the recombinant Salmonella resulted in the induction of V antigen-specific serum antibody responses and afforded protection against Y. pestis challenge. However, the antibody responses induced by the recombinant Salmonella did not correlate with the protection afforded, indicating that immune responses other than antibody may play a role in the protection afforded against plague by this candidate vaccine. Crown Copyright (C) 2003 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：3051 / 3057

页数：7

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