Isolation of new microsatellite loci from the Green Lizard (Lacerta viridis viridis)

被引:10
作者
Böhme, MU [1 ]
Berendonk, TU [1 ]
Schlegel, M [1 ]
机构
[1] Univ Leipzig, Inst Biol 2, Dept Mol Evolut & Anim Systemat, D-04103 Leipzig, Germany
来源
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 01期
关键词
Lacerta viridis; Lacertidae; microsatellites; polymorphism;
D O I
10.1111/j.1471-8286.2004.00825.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twelve new microsatellite loci were isolated from the Green Lizard (Lacerta viridis viridis). Primers for 28 loci were designed and 18 of these loci amplified well for 10 individuals of four populations. Twelve of these loci were further characterized for a population in Hungary. The results document the suitability of these identified loci for the characterization of the genetic diversity of the endangered species L. viridis viridis.
引用
收藏
页码:45 / 47
页数:3
相关论文
共 6 条
[1]   Modulation of non-templated nucleotide addition by taq DNA polymerase: Primer modifications that facilitate genotyping [J].
Brownstein, MJ ;
Carpten, JD ;
Smith, JR .
BIOTECHNIQUES, 1996, 20 (06) :1004-+
[2]  
ELBING K, 2001, CONTRIBUTIONS NATURA
[3]  
KALENDAR R, 2003, FASTPCR
[4]  
Peakall R., 2001, GENALEX V5 GENETIC A
[5]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[6]   Strategies for microsatellite isolation: a review [J].
Zane, L ;
Bargelloni, L ;
Patarnello, T .
MOLECULAR ECOLOGY, 2002, 11 (01) :1-16