Microbial Genomics as a guide to drug discovery and structural elucidation: ECO-02301, a novel antifungal agent, as an example

被引：175

作者：

McAlpine, JB ^{[1
]}

Bachmann, BO ^{[1
]}

Piraee, M ^{[1
]}

Tremblay, S ^{[1
]}

Alarco, AM ^{[1
]}

Zazopoulos, E ^{[1
]}

Farnet, CM ^{[1
]}

机构：

[1] Ecopia BioSci Inc, Montreal, PQ H4S 2A1, Canada

来源：

JOURNAL OF NATURAL PRODUCTS | 2005年 / 68卷 / 04期

关键词：

D O I：

10.1021/np0401664

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Analysis of the genome of Streptomyces aizunensis NRRL B-11277 indicated its potential to produce a compound of novel and highly predictable structure. The structure was predicted with sufficient accuracy to allow straightforward detection of the specific metabolite in HPLC profiles of fermentation extracts and hence to guide the isolation. The spectroscopic work was reduced to a confirmation of structure rather than a first principle determination. The compound, ECO-02301 (1), demonstrated potent antifungal activity. This work exemplifies not only the discovery of novel antibiotics from well-characterized organisms but also the utility of genomics as a further tool, complementary to spectroscopy, to enable rapid determination of complex structures.

引用

页码：493 / 496

页数：4

共 11 条

[1]

Isolation and structural elucidation of AC326-α, a new member of the moenomycin group [J].