Flux an important, but neglected, component of functional glenomics

被引:102
作者
Fernie, AR
Geigenberger, P
Stitt, M
机构
[1] Max Planck Inst Mol Pflanzenphysiol, Dept Metab Networks, D-14476 Golm, Germany
[2] Max Planck Inst Mol Pflanzenphysiol, Dept Mol Physiol, D-14476 Golm, Germany
关键词
D O I
10.1016/j.pbi.2005.01.008
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Genomics approaches aimed at understanding metabolism Currently tend to involve mainly expression profiling, although proteomics and steady-state metabolite profiling are increasingly being carried out as alternative strategies. These approaches provide rich information on the inventory of the cell. It is, however, of growing importance that such approaches are augmented by sophisticated integrative analyses and a higher-level understanding of cellular dynamics to provide insights into mechanisms that underlie biological processes. We argue the need for, and discuss theoretical and practical aspects of, the determination of metabolic flux as a component of functional genomics.
引用
收藏
页码:174 / 182
页数:9
相关论文
共 72 条
[1]   Carbocyclic fatty acids in plants:: Biochemical and molecular genetic characterization of cyclopropane fatty acid synthesis of Sterculia foetida [J].
Bao, XM ;
Katz, S ;
Pollard, M ;
Ohlrogge, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (10) :7172-7177
[2]   A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers [J].
Bologa, KL ;
Fernie, AR ;
Leisse, A ;
Loureiro, ME ;
Geigenberger, P .
PLANT PHYSIOLOGY, 2003, 132 (04) :2058-2072
[3]   Spatial analysis of plant metabolism:: Sucrose imaging within Vicia faba cotyledons reveals specific developmental patterns [J].
Borisjuk, L ;
Walenta, S ;
Rolletschek, H ;
Mueller-Klieser, W ;
Wobus, U ;
Weber, H .
PLANT JOURNAL, 2002, 29 (04) :521-530
[4]   PATH OF CARBON IN PHOTOSYNTHESIS [J].
CALVIN, M .
SCIENCE, 1962, 135 (3507) :879-&
[5]   Reduced expression of aconitase results in an enhanced rate of photosynthesis and marked shifts in carbon partitioning in illuminated leaves of wild species tomato [J].
Carrari, F ;
Nunes-Nesi, A ;
Gibon, Y ;
Lytovchenko, A ;
Loureiro, ME ;
Fernie, AR .
PLANT PHYSIOLOGY, 2003, 133 (03) :1322-1335
[6]   Improving intrinsic water-use efficiency and crop yield [J].
Condon, AG ;
Richards, RA ;
Rebetzke, GJ ;
Farquhar, GD .
CROP SCIENCE, 2002, 42 (01) :122-131
[7]   Functional genomics -: Silent genes given voice [J].
Cornish-Bowden, A ;
Cárdenas, ML .
NATURE, 2001, 409 (6820) :571-572
[8]   QUANTIFICATION OF COMPARTMENTED METABOLIC FLUXES IN MAIZE ROOT-TIPS USING ISOTOPE DISTRIBUTION FROM C-13-LABELED OR C-14-LABELED GLUCOSE [J].
DIEUAIDENOUBHANI, M ;
RAFFARD, G ;
CANIONI, P ;
PRADET, A ;
RAYMOND, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (22) :13147-13159
[9]   Contribution of malic enzyme, pyruvate kinase, phosphoenolpyruvate carboxylase, and the Krebs cycle to respiration and biosynthesis and to intracellular pH regulation during hypoxia in maize root tips observed by nuclear magnetic resonance imaging and gas chromatography mass spectrometry [J].
Edwards, S ;
Nguyen, BT ;
Do, B ;
Roberts, JKM .
PLANT PHYSIOLOGY, 1998, 116 (03) :1073-1081
[10]   Microdissection of histologic sections: Past, present, and future [J].
Eltoum, IA ;
Siegal, GP ;
Frost, AR .
ADVANCES IN ANATOMIC PATHOLOGY, 2002, 9 (05) :316-322