Optimization of headspace solid phase microextraction for gas chromatography/mass spectrometry analysis of widely different volatility and polarity terpenoids in olibanum

被引:81
作者
Hamm, S [1 ]
Lesellier, E [1 ]
Bleton, J [1 ]
Tchapla, A [1 ]
机构
[1] Inst Univ Technol Orsay, Grp Chim Analyt Paris Sud, LETIAM, EA 3343, F-91400 Orsay, France
关键词
olibanum; solid phase microextraction; boswellia; frankincense; headspace analysis; terpenoids; diterpenes; monoterpenes; Sesquiterpenes;
D O I
10.1016/j.chroma.2003.08.027
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was the optimization of headspace SPME conditions for trapping diterpenes present in frankincense (olibanum). Diterpenes like cembrenes or incensole and its derivatives are characteristic of olibanum. So in order to detect by SPME the occurrence of olibanum in archeological objects, it appears essential to have the best extraction conditions for these diterpenes that will be in very small quantities. Both sampling time and extraction temperature were studied and five fiber coatings were tested: polydimethylsiloxane (PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), carboxen/polydimethylsiloxane (CAR/PDMS), divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) and carbowax/divinylbenzene (CW/DVB). The PDMS/DVB fiber was found to be the most efficient for trapping olibanum characteristic diterpenes. with a sampling time of 1 h and a sampling temperature of 80degreesC. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:73 / 83
页数:11
相关论文
共 51 条
[1]  
Adams R. P., 1989, IDENTIFICATION ESSEN
[2]   Headspace solid phase microextraction. Dynamics and quantitative analysis before reaching a partition equilibrium [J].
Ai, J .
ANALYTICAL CHEMISTRY, 1997, 69 (16) :3260-3266
[3]   On-site field sampling and analysis of fragrance from living Lavender (Lavandula angustifolia L.) flowers by solid-phase microextraction coupled to gas chromatography and ion-trap mass spectrometry [J].
An, M ;
Haig, T ;
Hatfield, P .
JOURNAL OF CHROMATOGRAPHY A, 2001, 917 (1-2) :245-250
[4]  
[Anonymous], ENCY RELIG UNIVERS V
[5]   GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF CEMBRENOID DITERPENES IN KEROGEN FROM A LACUSTRINE SEDIMENT [J].
BARAKAT, AO ;
RULLKOTTER, J .
ORGANIC MASS SPECTROMETRY, 1993, 28 (03) :157-162
[6]  
Baratta MT, 1998, FLAVOUR FRAG J, V13, P235, DOI [10.1002/(SICI)1099-1026(1998070)13:4<235::AID-FFJ733>3.0.CO
[7]  
2-T, 10.1002/(SICI)1099-1026(1998070)13:4&lt
[8]  
235::AID-FFJ733&gt
[9]  
3.0.CO
[10]  
2-T]