Biodegradable poly(ether ester urethane)urea elastomers based on poly(ether ester) triblock copolymers and putrescine: synthesis, characterization and cytocompatibility

被引:208
作者
Guan, JJ
Sacks, MS
Beckman, EJ
Wagner, WR
机构
[1] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15261 USA
[3] Univ Pittsburgh, Dept Chem & Petr Engn, Pittsburgh, PA 15261 USA
关键词
biodegradable polymer; poly(ether ester urethane)urea; biodegradation; surface modification; cell compatibility;
D O I
10.1016/S0142-9612(03)00476-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Polymers with elastomeric mechanical properties, tunable biodegradation properties and cytocompatibility would be desirable for numerous biomedical applications. Toward this end a series of biodegradable poly(ether ester urethane)urea elastomers (PEEUUs) based on poly(ether ester) triblock copolymers were synthesized and characterized. Poly(ether ester) triblock copolymers were synthesized by ring-opening polymerization of P.-caprolactone with polyethylene glycol (PEG). PEEUUs were synthesized from these triblock copolymers and butyl diisocyanate, with putrescine as a chain extender. PEEUUs exhibited low glass transition temperatures and possessed tensile strengths ranging from 8 to 20 M Pa and breaking strains from 325% to 560%. Increasing PEG length or decreasing poly(caprolactone) length in the triblock segment increased PEEUU water absorption and biodegradation rate. Human umbilical vein endothelial cells cultured in a medium supplemented with PEEUU biodegradation solution suggested a lack of degradation product cytotoxicity. Endothelial cell adhesion to PEEUUs was less than 60% of tissue culture polystyrene and was inversely related to PEEUU hydrophilicity. Surface modification of PEEUUs with ammonia gas radio-frequency glow discharge and subsequent immobilization of the cell adhesion peptide Arg-Gly-Asp-Ser increased endothelial adhesion to a level equivalent to tissue culture polystyrene. These biodegradable PEEUUs thus possessed properties that would be amenable to applications where high strength and flexibility would be desirable and exhibited the potential for tuning with appropriate triblock segment selection and surface modification. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:85 / 96
页数:12
相关论文
共 42 条
[1]   THE PROPERTIES OF POLYURETHANES WITH MIXED CHAIN EXTENDERS AND MIXED SOFT SEGMENTS [J].
AHN, TO ;
JUNG, SU ;
JEONG, HM ;
LEE, SW .
JOURNAL OF APPLIED POLYMER SCIENCE, 1994, 51 (01) :43-49
[2]  
AN JH, 1999, J MATER SCI, V32, P726
[3]  
Bezemer JM, 2000, J BIOMED MATER RES, V52, P8, DOI 10.1002/1097-4636(200010)52:1<8::AID-JBM2>3.0.CO
[4]  
2-3
[5]   Synthesis and thermal properties of poly(ethylene glycol)-poly(ε-caprolactone) copolymers [J].
Bogdanov, B ;
Vidts, A ;
Van Den Bulcke, A ;
Verbeeck, R ;
Schacht, E .
POLYMER, 1998, 39 (8-9) :1631-1636
[6]   Functional tissue engineering: The role of biomechanics [J].
Butler, DL ;
Goldstein, SA ;
Guilak, F .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 2000, 122 (06) :570-575
[7]   Biodegradable poly(ethylene oxide)/poly(ε-caprolactone) multiblock copolymers [J].
Cohn, D ;
Stern, T ;
González, MF ;
Epstein, J .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 59 (02) :273-281
[8]   Segmented poly(ether-ester-amide)s based on poly(L,L-lactide) macromers [J].
D'Angelo, S ;
Galletti, P ;
Maglio, G ;
Malinconico, M ;
Morelli, P ;
Palumbo, R ;
Vignola, MC .
POLYMER, 2001, 42 (08) :3383-3392
[9]   SYNTHESIS AND CHARACTERIZATION OF PUTRESCINE-BASED POLY(PHOSPHOESTER-URETHANES) [J].
DAHIYAT, BI ;
HOSTIN, E ;
POSADAS, EM ;
LEONG, KW .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 1993, 4 (05) :529-543
[10]   Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage [J].
Davisson, T ;
Kunig, S ;
Chen, A ;
Sah, R ;
Ratcliffe, A .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2002, 20 (04) :842-848